A safer and relatively shorter method for Southern blot hybridization analysis

Journal of Biochemical and Biophysical Methods

Volumn 44, Issue 1-2, 2000, Pages 73-76

  Mergen, Hatice ^a   Acar, Ceren ^a   Birben, Esra ^a   Öner, Reyhan ^a   Öner, Cihan ^a  

^a HACETTEPE UNIVERSITY   (Turkey)

Author keywords

Polymerase chain reaction (PCR); Radioactive labelling; Southern blotting

Indexed keywords

DNA; DNA FRAGMENT; SULFUR 35;

ARTICLE; AUTORADIOGRAPHY; BLOOD SAMPLING; HUMAN; HUMAN CELL; HYBRIDIZATION; ISOTOPE LABELING; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SOUTHERN BLOTTING; TECHNIQUE;

BLOTTING, SOUTHERN; DNA; EQUIPMENT SAFETY; HUMANS; POLYMERASE CHAIN REACTION; TIME FACTORS;

EID: 0034632251     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-022X(00)00068-3     Document Type: Article

References (5)

1. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: A Laboratory Manual. 2nd Edition :1989;9.31-9.62 Cold Spring Harbor Laboratory Press, New York.
2. Southern E.M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 98:1975;503-517.
3. Saiki R.K., Gelfand D.H., Stoffel S., Scharf S.J., Higuchi R., Horn G.T., Mullis G.T. Primer directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 239:1988;487-494.
4. Zhou M.Y., Xue D., Gomez-Sanchez E.P., Gomez-Sanchez C.E. Improved downward capillary transfer for blotting of DNA and RNA. Biotechniques. 16:1994;58-59.
5. Yen M.Y., Yen T.C., Pang C.Y., Liu J.H., Wei Y.H. Mitochondrial DNA mutation in Leber's hereditary optic neuropathy. Invest Ophthalmol Vis Sci. 33:1992;2561-2566.