Quantitative microscopy after fluorescence in situ hybridization - A comparison between repeat-depleted and non-depleted DNA probes

Journal of Biochemical and Biophysical Methods

Volumn 44, Issue 1-2, 2000, Pages 59-72

  Rauch, Joachim ^a   Wolf, Dietmar ^a   Craig, Jeffrey M ^b   Hausmann, Michael ^a   Cremer, Christoph ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b UNIVERSITY OF MUNICH   (Germany)

Author keywords

Chromosome painting; Fluorescence in situ hybridization; Image analysis; Repeat depleted probes

Indexed keywords

ARTICLE; CHROMOSOME 15; CHROMOSOME PAINTING; DNA PROBE; FLUORESCENCE IN SITU HYBRIDIZATION; HUMAN; HUMAN CELL; IMAGE ANALYSIS; MICROSCOPY; PRIORITY JOURNAL;

CELL NUCLEUS; CHROMATOGRAPHY, AFFINITY; CHROMOSOMES, HUMAN, PAIR 15; DNA PROBES; GENE LIBRARY; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; MICROSCOPY; MICROSCOPY, VIDEO; POLYMERASE CHAIN REACTION; REPETITIVE SEQUENCES, NUCLEIC ACID; STREPTAVIDIN;

EID: 0034632224     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-022X(00)00047-6     Document Type: Article

References (22)

1. Bolzee A., Craig J.M., Cremer T., Speicher M.R. A complete set of repeat-depleted, PCR amplifiable, human chromosome-specific pointing probes. Cytogenit. Cell Genet. 84:1999;233-240.
2. Cremer T., Lichter P., Borden J., Ward D.C., Manuelidis L. Detection of chromosome aberrations in metaphase and interphase tumor cells by in situ hybridization using chromosome-specific library probes. Hum Genet. 80:1988;235-246.
3. Craig J.M., Kraus J., Cremer T. Removal of repetitive sequences from FISH probes using PCR-assisted affinity chromatography. Hum Genet. 100:1997;472-476.
4. Durm M., Haar F-M., Hausmann M., Ludwig H., Cremer C. Optimization of fast-fluorescence in situ hybridization with repetitive α-satellite probes. Z Naturforsch. 51c:1996;253-261.
5. Durm M., Hausmann M., Cremer C. Painting of human chromosome 8 in fifteen minutes. Z. Naturforsch. 51c:1996;435-439.
6. Durm M., Schüssler L., Münch H., Craig J., Ludwig H., Hausmann M., Cremer C. Fast-painting of human metaphase spreads using a chromosome specific repeat depleted DNA library probe. Biotechniques. 24:1998;820-825.
7. Florijn R.J., Bonden L.A.J., Vrolijk H., Wiegant J., Vaandrager J.W., Baas F., Dunnen J.T., denTanke H.J., Ommen G.J.B., van Raap A.K. High-resolution DNA fiber-FISH for genomic DNA mapping and colour bar-coding of large genes. Hum Mol Genet. 4:1995;831-836.
8. Guan X-Y., Meltzer P.S., Trent J.M. Rapid generation of whole chromosome painting probes (WCPs) by chromosome microdissection. Genomics. 22:1994;101-107.
9. Haar F-M., Durm M., Hausmann M., Ludwig H., Cremer C. Optimization of fast-FISH for α-satellite DNA probes. J Biochem Biophys Meth. 33:1996;43-54.
10. Joos S., Scherthan H., Speicher M.R., Schlegel J., Cremer T., Lichter P. Detection of amplified DNA sequences by reverse chromosome painting using genomic tumor DNA as probe. Hum Genet. 90:1993;584-589.
11. Kallionemi A., Kallionemi O.P., Sudar D., Rutovitz D., Gray J.W. Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors. Science. 258:1992;818-821.
12. Lichter P., Cremer T., Borden J., Manuelidis L., Ward D.C. Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries. Hum Genet. 80:1988;224-234.
13. Lichter P., Cremer T. Chromosome analysis by non-isotopic in situ hybridization. Rooney D.E., Czepulkowski B.H. 2nd Edition Human Cytogenetics - A practical approach. Vol. I:1992;157-192 IRL Press, Oxford.
14. du Manoir S., Speicher M.R., Joos S., Schröck E., Popp S., Döhner H., Kovacs G., Robert-Nicoud M., Lichter P., Cremer T. Detection of complete and partial chromosome gains and losses by comparative genomic in situ hybridization. Hum Genet. 90:1993;590-610.
15. Pinkel D., Landegent J., Collins C., Fuscoe J., Segraves R., Lucas J., Gray J.W. Fluorescence in situ hybridization with human chromosome-specific libraries: detection of trisomy 21 and translocations of chromosome 4. Proc Natl Acad Sci USA. 85:1988;9138-9142.
16. Ried T., Mahler V., Vogt P., Blonden L., van Ommen G.J., Cremer T., Cremer M. Direct carrier detection by in situ suppression hybridization with cosmid clones of the Duchenne/Becker muscular dystrophy locus. Hum Genet. 85:1990;581-586.
17. Roquier S., Trask B.J., Taviaux S., vandenEngh G., Diriong S., Lennon G.G., Giorgi D. Direct selection of cDNAs using whole chromosomes. Nucl. Acids Res. 23:1995;4415-4420.
18. Sambrook J., Fritsch E.F., Maniatis T. Molecular cloning - a laboratory manual. 2nd Edition :1989;Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
19. Schardin M., Cremer T., Hager H.D., Lang M. Specific staining of human chromosomes in Chinese hamster×man hybrid cell lines demonstrates interphase chromosome territories. Hum Genet. 71:1985;281-287.
20. Schröck E., du Manoir S., Veldman T., Schoell B., Wienberg J., Ferguson-Smith M.A., Ning Y., Ledbetter D.H., Bar-Am I., Soenksen D., Garini Y., Ried T. Multicolor spectral karyotyping of human chromosomes. Science. 273:1996;494-497.
21. Speicher M.R., Ballard S.G., Ward D.C. Karyotyping by combinatorial multi-fluor FISH. Nature Genet. 12:1996;368-375.
22. Telenius H., Pelmear A.H., Tunnacliffe A., Carter N.P., Behmel A., Ferguson-Smith M.A., Nordenskjöld M., Pfragner R., Ponder B.A.J. Cytogenetic analysis by chromosome painting using DOP-PCR amplified flow-sorted chromosomes. Genes Chrom Cancer. 4:1992;257-263.