The major HIV-1 packaging signal is an extended bulged stem loop whose structure is altered on interaction with the Gag polyprotein

Journal of Molecular Biology

Volumn 297, Issue 4, 2000, Pages 877-893

  Zeffman, Amanda ^a   Hassard, Stuart ^b   Varani, Gabriele ^a   Lever, Andrew ^b  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

HIV; NMR; Packaging; RNA; RNA protein

Indexed keywords

GAG POLYPROTEIN; PROTEIN; RNA; UNCLASSIFIED DRUG;

ARTICLE; CONFORMATIONAL TRANSITION; HUMAN IMMUNODEFICIENCY VIRUS 1; MOLECULAR STABILITY; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN RNA BINDING; RNA PROCESSING; RNA STRUCTURE; STRUCTURE ANALYSIS;

ANIMALIA; DNA VIRUSES; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 0034615776     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.3611     Document Type: Article

References (45)

1. Mutations of RNA and protein sequences involved in human immunodeficiency virus type 1 packaging result in production of noninfectious virus
2. Divalent metal ion binding to a conserved wobble pair defining the upstream site of cleavage of group I self-splicing introns
3. Structure and function of the human immunodeficiency virus leader RNA
4. Analysis of binding elements in the human immunodeficiency virus type 1 genomic RNA and nucleocapsid protein
5. Specific binding of human immunodeficiency virus type 1 gag polyprotein and nucleocapsid protein to viral RNAs detected by RNA mobility shift assays
6. 5' Regions of HIV-1 RNAs are not sufficient for encapsidation: Implications for the HIV-1 packaging signal
7. Solution structure of the loop B domain from the hairpin ribozyme
8. In vitro assembly of virus-like particles with Rous sarcoma virus Gag deletion mutants: Identification of the p10 domain as a morphological determinant in the formation of spherical particles
9. Evidence for 30-40S RNA as precursor of the 60-70S RNA of Rous sarcoma virus
10. A mutant of human immunodeficiency virus with reduced RNA packaging and abnormal particle morphology
11. RNA secondary structure and binding sites for gag gene products in the 5' packaging signal of human imunodeficiency virus type 1
12. Mutant human immunodeficiency virus type 1 genomes with defects in RNA dimerization or encapsidation
13. Mapping the RNA binding sites for human immunodeficiency virus type 1 Gag and NC proteins within the complete HIV-1 and -2 untranslated leader regions
14. Cis elements and trans-acting factors involved in the RNA dimerization of the human immunodeficiency virus HIV-1
15. Structure of the HIV-1 nucleocapsid protein bound to the SL3 psi-RNA recognition element
16. HIV-1 nucleocapsid protein induces 'maturation' of dimeric retroviral RNA in vitro
17. Maturation of dimeric viral RNA of Moloney murine leukaemia virus
18. Retroviral RNA dimer linkage
19. The human immunodeficiency virus type 1 packaging signal and major splice donor region have a conserved stable secondary structure
20. Secondary structure model of the Mason-Pfizer monkey virus 5' leader sequence: Identification of a structural motif common to a variety of retroviruses
21. Pausing of reverse transcriptase on retroviral RNA templates is influenced by secondary structures both 5' and 3' of the catalytic site
22. Functional analysis of the core packaging signal in a permissive cell line
23. Trans-acting proteins involved in RNA encapsidation and viral assembly in human immunodeficiency virus type 1
24. Non-reciprocal packaging of human immunodeficiency virus type 1 and type 2 RNA: A possible role for the p2 domain of Gag in RNA encapsidation
25. Human immunodeficiency virus types 1 and 2 use different mechanisms for selection of unspliced RNA for encapsidation
26. Recombinant human immunodeficiency virus type 1 nucleocapsid (NCpand) protein unwinds tRNA
27. Mutations in the kissing-loop hairpin of human immunodeficiency virus type 1 reduce viral infectivity as well as genomic RNA packaging and dimerization
28. Identification of a sequence required for efficient packaging of human immunodeficiency virus type 1 RNA into virions
29. Human immunodeficiency virus type-1 nucleocapsid protein (NCp7) directs specific initiation of minus-strand DNA synthesis primed by human tRNA(3)(Lys) in-vitro: Studies of viral RNA molecules mutated in regions that flank the primer binding site
30. Retroviral RNA packaging: Sequence requirements and implications
31. A molecular rheostat. Co-operative rev binding to stem I of the revresponse element modulates human immunodeficiency virus type-1 late gene expression
32. 1H assignments
33. The human immunodeficiency virus type 1 encapsidation site is a multipartite RNA element composed of functional hairpin structures
34. Location of cisacting signals important for RNA encapsidation in the leader sequence of human immunodeficiency virus type 2
35. NCp7 activates H1V-1Lai RNA dimerization by converting a transient loop-loop complex into a stable dimer
36. Three-dimensional folding of an RNA hairpin required for packaging HIV-1
37. Preparation of RNA-protein complexes for X-ray crystallography and NMR
38. Synthesis, assembly and processing of viral protein
39. The conformation of the sarcin/ricin loop from 28S ribosomal RNA
40. The tobacco mosaic virus assembly origin RNA. Functional characteristics defined by directed mutagenesis
41. NMR investigation of RNA structure
42. Form, function, and use of retroviral Gag proteins
43. The conformation of loop E of eukaryotic 55 ribosomal RNA
44. Human immunodeficiency virus type 1 nucleocapsid protein reduces reverse transcriptase pausing at a secondary structure near the murine leukemia virus polypurine tract
45. Recognition of a tetranucleotide loop of signal recognition particle RNA by protein SRP19