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Volumn 10, Issue 3, 2000, Pages 261-264

Synthesis and evaluation of daunorubicin-paclitaxel dimers

Author keywords

[No Author keywords available]

Indexed keywords

DAUNORUBICIN; DIMER; DNA; MICROTUBULE PROTEIN; PACLITAXEL;

EID: 0034615062     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-894X(99)00667-8     Document Type: Article
Times cited : (15)

References (22)
  • 10
    • 0004260607 scopus 로고
    • Ed.; Academic Press: New York
    • Doxorubicin; Arcamone, F., Ed.; Academic Press: New York, 1981.
    • (1981) Doxorubicin
    • Arcamone, F.1
  • 13
    • 84991411298 scopus 로고    scopus 로고
    • note
    • 13C); 5c: calcd 1582, found 1605 (M + Na); 5d: calcd 1582, found 1605 (M+Na); 5e: calcd 1562, found 1563 (MH+); 5f: calcd 1560, found 1583 (M+Na).
  • 15
    • 84991418713 scopus 로고    scopus 로고
    • note
    • 3H]PTX (5 μgCi/μmol, Moravek Biochemical) and other competing drugs were added to the solution containing the assembled MTs and incubated for an additional 45 min at 37 °C. The MTs were then pelleted by centrifugation at 14,000 rpm in an Eppendorf microfuge for 30 min. The supernatants were carefully removed and the pellets were resuspended in 0.1 M NaOH solution then neutralized with 0.1 M HCl and transferred to a scintillation vial. Approximately 5 mL of Cytoscint liquid scintillation cocktail was added to each, and the samples were counted on a Beckman LS 3801 Liquid Scintillation Counter.
  • 16
    • 84991411299 scopus 로고    scopus 로고
    • note
    • UV-visible absorption spectra were measured for solutions of DNR (0-50 μM). Next, tubulin (1.0 mg/mL) was treated with varying amounts of DNR (0-50 μM) in either the presence or absence of PTX (10 μM). All protein containing samples were pelleted by centrifugation, and the supernatants were analyzed spectrophotometrically. The absorption at 480 nm for all solutions was plotted as a function of DNR concentration.
  • 18
    • 84991427399 scopus 로고    scopus 로고
    • note
    • 50. Values reported for PTX + DNR combination are the concentrations of each compound for a total drug concentration twice the reported value.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.