Membrane proteins PmpG and PmpH are major constituents of Chlamydia trachomatis L2 outer membrane complex

FEMS Microbiology Letters

Volumn 186, Issue 2, 2000, Pages 163-169

  Mygind, Per Holse ^a   Christiansen, Gunna ^a   Roepstorff, Peter ^b   Birkelund, Svend ^a  

^a AARHUS UNIVERSITY   (Denmark)

^b UNIVERSITY OF SOUTHERN DENMARK   (Denmark)

Author keywords

Chlamydia; Mass spectrometry; Membrane protein; Sodium dodecyl sulfate polyacrylamide gel electrophoresis

Indexed keywords

BACTERIAL PROTEIN; OUTER MEMBRANE PROTEIN;

ARTICLE; BACTERIAL OUTER MEMBRANE; BACTERIUM ADHERENCE; CHLAMYDIA TRACHOMATIS; CONTROLLED STUDY; HOST CELL; LASER; MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; SILVER STAINING;

AMINO ACID SEQUENCE; BACTERIAL OUTER MEMBRANE PROTEINS; CHLAMYDIA TRACHOMATIS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PEPTIDE FRAGMENTS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TRYPSIN;

CHLAMYDIA; CHLAMYDIA TRACHOMATIS;

EID: 0034607779     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(00)00135-X     Document Type: Article

References (22)

1. Schachter, J. (1999) Infection and disease epidemiology. In: Chlamydia; Intracellular Biology, Pathogenesis, and Immunity (Stephens, R.S., Ed.), pp. 139-169. American Society of Microbiology, Washington, DC.
2. Stephens R.S., Kalman S., Lammel C., Fan J., Marathe R., Aravind L., Mitchell W., Olinger L., Tatusov R.L., Zhao Q., Koonin E.V., Davis R.W. Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis. Science. 282:1998;754-759.
3. Kalman S., Mitchell W., Marathe R., Lammel C., Fan J., Hyman R.W., Olinger L., Grimwood J., Davis R.W., Stephens R.S. Comparative genomes of Chlamydia pneumoniae and C. trachomatis. Nature Genet. 21:1999;385-389.
4. Struyve M., Moons M., Tommassen J. Carboxy-terminal phenylalanine is essential for the correct assembly of a bacterial outer membrane protein. J. Mol. Biol. 218:1991;141-148.
5. Caldwell H.D., Kromhout J., Schachter J. Purification and partial characterization of the major outer membrane protein of Chlamydia trachomatis. Infect. Immun. 31:1981;1161-1176.
6. Hatch, T.P. (1999) Developmental biology. In: Chlamydia; Intracellular Biology, Pathogenesis, and Immunity (Stephens, R.S., Ed.), pp. 29-67. American Society of Microbiology, Washington, DC.
7. Stothard D.R., Boguslawski G., Jones R.B. Phylogenetic analysis of the Chlamydia trachomatis major outer membrane protein and examination of potential pathogenic determinants. Infect. Immun. 66:1998;3618-3625.
8. Longbottom D., Russell M., Jones G.E., Lainson F.A., Herring A.J. Identification of a multigene family coding for the 90 kDa proteins of the ovine abortion subtype of Chlamydia psittaci. FEMS Microbiol. Lett. 142:1996;277-281.
9. Longbottom D., Findlay J., Vretou E., Dunbar S.M. Immunoelectron microscopic localisation of the OMP90 family on the outer membrane surface of Chlamydia psittaci. FEMS Microbiol. Lett. 164:1998;111-117.
10. Knudsen K., Madsen A.S., Mygind P., Christiansen G., Birkelund S. Identification of two novel genes encoding 97- to 99-kilodalton outer membrane proteins of Chlamydia pneumoniae. Infect. Immun. 67:1999;375-383.
11. Longbottom D., Russell M., Dunbar S.M., Jones G.E., Herring A.J. Molecular cloning and characterization of the genes coding for the highly immunogenic cluster of 90-kilodalton envelope proteins from the Chlamydia psittaci subtype that causes abortion in sheep. Infect. Immun. 66:1998;1317-1324.
12. Jensen O.N., Larsen M.R., Roepstorff P. Mass spectrometric identification and microcharacterization of proteins from electrophoretic gels: Strategies and applications. Proteins Struct. Funct. Genet. 999:(Proteins Suppl. 2):1998;74-89.
13. Birkelund S., Lundemose A.G., Christiansen G. Chemical crosslinking of Chlamydia trachomatis. Infect. Immun. 56:1988;654-659.
14. Nesterenko M.V., Tilley M., Upton S.J. A simple modification of Blum's silver stain method allows for 30 minute detection of proteins in polyacrylamide gels. J. Biochem. Biophys. Methods. 28:1994;239-242.
15. Rosenfeld J., Capdevielle J., Guillemot J.C., Ferrara P. In-gel digestion of proteins for internal sequence analysis after one- or two-dimensional gel electrophoresis. Anal. Biochem. 203:1992;173-179.
16. Lindquist, E.A. and Stephens, R.S. (1998) Transcriptional activity of a sequence variable protein family in Chlamydia trachomatis. In: Chlamydial Infections: Proceedings of the Ninth International Symposium on Human Chlamydial Infection. International Chlamydia Symposium (Stephens, R.S. et al., Eds), pp. 259-262.
17. Altschul S.F., Madden T.L., Schäffer A.A., Zhang J., Zhang Z., Miller W., Lipman D.J. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:1997;3389-3402.
18. Everett K.D.E., Hatch T.P. Architecture of the cell envelope of Chlamydia psittaci 6BC. J. Bacteriol. 177:1995;877-882.
19. Buendia A.J., Salinas J., Sanchez J., Gallego M.C., Rodolakis A., Cuello F. Localization by immunoelectronmicroscopy of antigens of Chlamydia psittaci suitable for diagnosis or vaccine development. FEMS Microbiol. Lett. 150:1997;113-119.
20. Newhall W.J., Jones R.B. Disulfide-linked oligomers of the major outer membrane protein of Chlamydiae. J. Bacteriol. 154:1983;998-1001.
21. Salari S.H., Ward M.E. Polypeptide composition of Chlamydia trachomatis. J. Gen. Microbiol. 123:1981;197-207.
22. Ramsey K.H., Cotter T.W., Salyer R.D., Miranpuri G.S., Yanez M.A., Poulsen C.E., DeWolfe J.L., Byrne G.I. Prior genital tract infection with a murine or human biovar of Chlamydia trachomatis protects mice against heterotypic challenge infection. Infect. Immun. 67:1999;3019-3025.