Cultivation of immortalized human hepatocytes HepZ on macroporous CultiSpher G microcarriers

Biotechnology and Bioengineering

Volumn 68, Issue 1, 2000, Pages 59-70

  Werner, Andreas ^a   Duvar, Sevim ^a   Müthing, Johannes ^a   Büntemeyer, Heino ^a   Lünsdorf, Heinrich ^b   Strauss, Michael ^c   Lehmann, Jürgen ^a  

^a BIELEFELD UNIVERSITY   (Germany)

^b HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^c MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

Author keywords

Amino acid metabolism; Bioartificial liver; Cell line; Hypothermia; Immortalized hepatocytes; Oxygen consumption; Spinner cultivation; Stirred bioreactor

Indexed keywords

AMINO ACID; AROMATIC AMINO ACID; BRANCHED CHAIN AMINO ACID;

AMINO ACID METABOLISM; ARTICLE; ARTIFICIAL LIVER; ARTIFICIAL ORGAN; BIOREACTOR; CELL IMMORTALIZATION; ENZYME ASSAY; HUMAN; HUMAN CELL; HYPOTHERMIA; LIVER CELL; OXYGEN CONSUMPTION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

AMINO ACIDS, BRANCHED-CHAIN; ANESTHETICS, LOCAL; BIOREACTORS; BIOTRANSFORMATION; CELL CULTURE TECHNIQUES; CELL DIVISION; CELL LINE, TRANSFORMED; COLD; CULTURE MEDIA; HUMANS; L-LACTATE DEHYDROGENASE; LIDOCAINE; LIVER; LIVER, ARTIFICIAL; MICROSCOPY, ELECTRON; MICROSCOPY, ELECTRON, SCANNING; MICROSPHERES; OXYGEN CONSUMPTION; PHENYLALANINE; TYROSINE;

EID: 0034607270     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(20000405)68:1<59::AID-BIT7>3.0.CO;2-N     Document Type: Article

References (35)

1. Büntemeyer H, Lütkemeyer D, Lehmann J. 1991. Optimization of serum-free fermentation processes for antibody production. Cytotechnology 5:57-67.
2. Büntemeyer, H. Methods for off-line analysis in animal cell culture. In: Spier RE, editor. The Encyclopedia of Cell Technology. New York: Wiley. In press.
3. Duvar S, Müthing J, Mohn H, Lehmann J. 1996. Scale up cultivation of primary human umbilical vein endothelial cells on microcarriers from spinner vessels to bioreactor fermentation. Cytotechnology 21:61-72.
4. Flendrig LM. 1999. Evaluation of a novel design bioartificial liver in rats with complete liver ischemia: Treatment efficiacy and species-specific α-GST detection as a first attempt to monitor hepatocyte viability. J Hepatol 30:311-320.
5. Gerlach JC. 1994. Hybrid liver support system in a short-term application on hepatectomized pigs. Int J Artif Org 17:549-553.
6. Gerlach JC. 1996. Development of a hybrid liver support system: A review. Int J Artif Organs 19:645-654.
7. Hodgson HJF, Cunningham JM. 1992. Microcarrier culture of hepatocytes in whole plasma for use in liver support bioreactors. Int J Artif Organs 15:162-167.
8. Jauregui HO, Chowdhury NR, Chowdhury JR. 1996. Use of mammalian liver cells for artificial liver support. Cell Transplant 5:353-367.
9. Kasai SI, Sawa M, Mito M. 1994. Is the biological artificial liver clinically applicable? A historic review of biological artificial liver support systems. Artif Organs 18:348-354.
10. Kirillowa I. 1996. Etablierung teilungsfähiger differenzierter Hepatozyten der Maus und des Menschen durch genetische Beeinflussung der Zellteilungsregulation. Ph.D. thesis, Humboldt University, Berlin, Germany.
11. Kono Y, Yang S, Letarte M, Roberts EA. 1995. Establishment of a human hepatocyte line derived from primary culture in a collagen gel sandwich culture system. Exp Cell Res 221:478-485.
12. Lehmann J, Piehl GW, Schulz R. 1987. Bubble-free cell culture aeration with porous moving membranes. Dev Biol Stand 66:227-240.
13. Lehmann S, Vorlop J, Büntemeyer H. 1988. Bubble-free reactors and their development for continuous culture with cell recycle. In: Spier RE, Griffiths JB, editors. Animal Cell Biotechnology. 3rd ed. New York: Academic Press. p 221-237.
14. Matthew HWT, Basu S, Peterson WD, Salley SO, Klein MD. 1993. Performance of plasma-perfused, microencapsulated hepatocytes: Prospects for extracorporeal liver support. J Pediatr Surg 28:1423-1428.
15. Müthing J, Duvar S, Nerger S, Büntemeyer H, Lehmann J. 1996. Micro-carrier cultivation of bovine aortic endothelial cells in spinner vessels and a membrane stirred bioreactor. Cytotechnology 18:193-206.
16. Naik S, Santangini HA, Trenkler DM, Mullon CJ-P, Solomon BA, Pan J, Jauregui HO. 1997. Functional recovery of porcine hepatocytes after hypothermie or cryogenic preservation for liver support systems. Cell Transplant 6:447-454.
17. Neuzil DF, Rozga J, Moscioni AD, Ro MS, Hakim R, Arnaout WS, Demetriou A. 1993. Use of a novel bioartificial liver in a patient with acute liver insufficiency. Surgery 113:340-343.
18. Nyberg SL, Remmel RP, Mann HJ, Peshwa MV, Hu WS, Cerra FB. 1994. Primary hepatocytes outperform Hep G2 cells as the source of biotransformation functions in a bioartificial liver. Ann Surg 226:59-67.
19. Nyberg SL, Shatford RA, Peshwa MV, White JG, Cerra FB, Hu WS. 1993. Evaluation of a hepatocyte-entrapment hollow fiber bioreactor: A potential bioartificial liver. Biotechnol Bioeng 41:194-203.
20. Oellerich M, Burdelski M, Ringe B, Lamesch P, Gubernatis G, Bunzendahl H, Pichlmayr R, Herrmann H. 1989. Lignocaine metabolite formation as measure of pre-transplant liver function. Lancet 25:640-642.
21. Oellerich M, Raude E, Burdelski M, Schulz M, Schmidt FW, Ringe B, Lamesch P, et al. 1987. Monoethylglycinexylidide formation kinetics: A novel approach to assessment of liver function. J Clin Chem Clin Biochem 25:845-853.
22. Patience C, Takeuchi Y, Weiss RA. 1997. Infection of human cells by endogenous retrovirus of pigs. Nature Med 3:282-286.
23. Reynolds ES. 1963. The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J Cell Biol 17:208-212.
24. Rozga J, Holzman MD, Ro MS, Griffin DW, Neuzil DF, Giorgio T, Moscioni AD, Demetriou A. 1993. Development of a hybrid bioartificial liver. Ann Surg 217:502-511.
25. Rozga J, Morsiani E, LePage E, Moscioni AD, Giorgio T, Demetriou AA. 1994. Isolated hepatocytes in a bioartificial liver: A single group view and experience. Biotechnol Bioeng 43:645-653.
26. Seglen PO. 1973. Preparation of rat liver cells. Exp Cell Res 82:391-398.
27. Shatford RA, Nyberg SL, Meier SJ, White JG, Payne WD, Hu WS, Cerra FB. 1992. Hepatocyte function in a hollow fiber bioreactor: A potential bioartificial liver. J Surg Res 53:549-557.
28. Shirara H, Koide N, Hada H, Ujike K, Nakamura M, Shinji T, Gotoh S, Tsuji T. 1996. Improvement of serum amino acid profile in hepatic failure with the bioartificial liver using multicellular hepatocyte spheroids. Biotechnol Bioeng 50:416-421.
29. Spurr AR. 1969. A low viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26:31-43.
30. Strauss M, Griffin BE. 1990. Cellular immortalization: An essential step or merely a risk factor in DNA virus-induced transformation? Cancer Cells 2:360-365.
31. Sussman NL, Chong MG, Koussayer T, He DE, Shang TA, Whisennand HH, Kelly JH. 1992. Reversal of fulminant hepatic failure using an extracorporeal liver assist device. Hepatology 16:60-65.
32. Sussman NL, Gislason GT, Kelly JH. 1994. Extracorporal liver support. Application to fulminant hepatic failure. J Clin Gastroenterol 18: 320-324.
33. Takahashi M, Ishikura H, Takahashi C, Nakajima Y, Matsushita M, Matsue H, Sato K, et al. 1993. Immunologic considerations in the use of cultured porcine hepatocytes as a hybrid artificial liver. ASAIO J 242-246.
34. Uchino J, Matsue H, Takahashi M, Nakajima Y, Matsushita M, Hamada T, Hashimura E. 1991. Function of cultured monolayer pig hepatocytes in plasma from hepatic failure patients. Trans Am Soc Artif Intern Organs 37:337-338.
35. Yarmush ML, Dunn JCY, Tompkins RG. 1992. Assessment of artificial liver support technology. Cell Transplant 1:323-341.