Matrix-mixed culture: New methodology for chondrocyte culture and preparation of cartilage transplants

Journal of Biomedical Materials Research

Volumn 49, Issue 3, 2000, Pages 305-311

  Perka, Carsten ^a   Spitzer, Ron Sascha ^a   Lindenhayn, Klaus ^a   Sittinger, Michael ^a   Schultz, Olaf ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

Alginate; Cartilage transplantation; Cell culture; Fibrin glue; Tissue engineering

Indexed keywords

BIOCHEMISTRY; CARTILAGE; ELECTRON MICROSCOPY; IMMUNOLOGY; MORPHOLOGY; POLYSACCHARIDES; TISSUE CULTURE; TRANSPLANTATION (SURGICAL);

HISTOMORPHOLOGY; IMMUNOHISTOCHEMISTRY;

BIOMATERIALS;

ALGINIC ACID; BIOMATERIAL; FIBRIN;

ARTICLE; ARTICULAR CARTILAGE; BIOENGINEERING; CARTILAGE CELL; CARTILAGE MATRIX; CARTILAGE TRANSPLANTATION; CELL CULTURE; CELL DIFFERENTIATION; CELL PROLIFERATION; CONTROLLED STUDY; ELECTRON MICROSCOPY; EMBEDDING; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; METHODOLOGY;

ALGINATES; BIOCOMPATIBLE MATERIALS; BIOMEDICAL ENGINEERING; CARTILAGE, ARTICULAR; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL DIVISION; CHONDROCYTES; COLLAGEN; FIBRIN; GLUCURONIC ACID; HEXURONIC ACIDS; HUMANS; IMMUNOHISTOCHEMISTRY; MATERIALS TESTING; MICROSCOPY, ELECTRON; PROTEOGLYCANS;

EID: 0034607177     PISSN: 00219304     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-4636(20000305)49:3<305::AID-JBM2>3.0.CO;2-9     Document Type: Article

References (31)

1. Buschmann MD, Gluzband YA, Grodzinsky AJ, Kimura JH, Hunzicker EB. Mechanical compression modulates matrix biosynthesis in chondrocyte/agarose gel culture. In: Proceedings of the Combined Meeting of the Orthopedic Research Society of the USA, Japan, and Canada. 1991.
2. Kimura T, Yasui N, Ohsawa S, Ono K. Biosynthesis of type IX collagen during chick limb development. Biochem Biophys Res Commun 1985;130:746-752.
3. Homminga GN, Buma AP, Koot HW, van der Kraan PM, van den Berg WB. Chondrocyte behavior in fibrin glue in vitro. Acta Orthop Scand 1993;64:441-445.
4. Häuselmann HJ, Aydelotte MB, Schumacher BL, Kuettner KE, Gitelis SH, Thonar EJ. Synthesis and turnover of proteoglycans by human and bovine adult articular chondrocytes cultured in alginate beads. Matrix 1992;12:116-129.
5. Häuselmann HJ, Fernandes RJ, Mok SS, Schmid TM, Block JA, Aydelotte MB, Kuettner KE, Thonar EJ. Phenotypic stability of bovine articular chondrocytes after long-term culture in alginate beads. J Cell Sci 1994;107:17-27.
6. Puelacher WC, Mooney D, Langer R, Upton J, Vacanti JP, Vacanti CA. Design of nasoseptal cartilage replacements synthesized from biodegradable polymers and chondrocytes. Biomaterials 1994;15:774-778.
7. Sittinger M. Tissue engineering: Künstlicher gewebeersatz aus vitalen xkomponenten. Laryngorhinootologie 1995;74:695-699.
8. Sittinger M, Bujia J, Minuth WW, Hammer C, Burmester GR. Engineering of cartilage tissue using bioresorbable polymer carriers in perfusion culture. Biomaterials 1994;15:451-456.
9. Sittinger M, Bujia J, Rotter N, Reitzel D, Minuth WW, Burmester GR. Tissue engineering and autologous transplant formation: practical approaches with resorbable biomaterials and new cell culture techniques. Biomaterials 1996;17:237-242.
10. Vacanti CA, Vacanti JP. Bone and cartilage reconstruction with tissue engineering approaches. Otolaryngol Clin North Am 1994;27:263-276.
11. Martinsen A, Skjak-Braek G, Smidsrod O. Alginate as immobilisation material: 1. Correlation between chemical and physical properties of alginate gel beads. Biotechnol Bioeng 1989;33: 79.
12. Soon-Shiong P, Feldman E, Nelson R, Heintz R, Yao Q, Yao Z, Zheng T, Merideth N, Skjak-Braek G, Espevik T, Smidsrod O, Sandford P. Long-term reversal of diabetes by the injection of immunoprotected islets. Proc Natl Acad Sci USA 1993;90:5843-5847.
13. Soon-Shiong P, Otterlei M, Skjak-Braek G. An immunologic basis for the fibrotic reaction to implanted micro-capsules. Transplant Proc 1991;23:758-759.
14. Espevik T, Otterlei M, Skjak-Braek G, Ryan L, Wright SD, Sundan A. The involvement of CD14 in stimulation of cytokine production by uronic acid polymers. Eur J Immunol 1993;23: 255-261.
15. Otterlei M, Espevik T, Ostgaard K, Skjak-Braek G, Soon-Shiong P, Smidsrod O. Induction of cytokine production from human monocytes stimulated with alginate. J Immunother 1991;10:286-291.
16. Staindl O, Galvan O. Zur resorption des fibrinklebers nach plastischen operationen im gesichtsbereich. Eine untersuchung mit radioaktiven isotopen. In: Fibrinkleber in orthopädie und traumatologie. Cotta H, Braun A, editors. Stuttgart: Thieme; 1982. p 11-14.
17. Scheele J, Pesch HJ. Morphologische aspekte des fibrinkleberabbaus im tierexperiment. In: Fibrinkleber in orthopädie und traumatologie. Cotta H, Braun A, editors. Stuttgart: Thieme; 1982. p 35-42.
18. Keller J, Andreassen TT, Joyce F, Knudsen VE, Jorgensen PH, Lucht U. Fixation of osteochondral fractures. Fibrin sealant tested in dogs. Acta Orthop Scand 1985;56:323-326.
19. Henning CE, Lynch MA, Yearout KM, Vequist SW, Stallbaumer RJ, Decker KA. Arthroscopic meniscal repair using an exogenous fibrin clot. Clin Orthop 1990;252:64-72.
20. Hendrickson DA, Nixon AJ, Erb HN, Lust G. Phenotype and biological activity of neonatal equine chondrocytes cultured in a three-dimensional fibrin matrix. Am J Vet Res 1994;55:410-414.
21. Alsalameh S, Mollenhauer J, Hain N, Stock KP, Kalden JR, Burmester GR. Cellular immune response toward human articular chondrocytes. T cell reactivities against chondrocyte and fibroblast membranes in destructive joint diseases. Arthritis Rheum 1990;33:1477-1486.
22. Elves MW, Zervas J. An investigation into the immunogenicity of various components of osteoarticular grafts. Br J Exp Pathol 1974;55:344-351.
23. Heyner S. The antigenicity of cartilage grafts. Surg Gynecol Obstet 1973;136:298-305.
24. Langer F, Gross AE, Greaves MF. The auto-immunogenicity of articular cartilage. Clin Exp Immunol 1972;12:31-37.
25. Balazs EA, Bland PA, Denlinger JL, Goldman AI, Larsen NE, Leshchiner EA, Leshchiner A, Morales B. Matrix engineering. Blood Coagul Fibrinolysis 1991;2:173-178.
26. Ramdi H, Legay C, Lievremont M. Influence of matricial molecules on growth and differentiation of entrapped chondrocytes. Exp Cell Res 1993;207:449-454.
27. Aydelotte MB, Thonar EJ, Mollenhauer J, Flechtenmacher J. Culture of chondrocytes in alginate gel: variations in conditions of gelation influence the structure of the alginate gel, and the arrangement and morphology of proliferating chondrocytes. In Vitro Cell Dev Biol Anim 1998;34:123-130.
28. Hoffstrom BG, Wayner EA. Immunohistochemical techniques to study the extracellular matrix and its receptors. Methods Enzymol 1994;245:316-346.
29. Guo JF, Jourdin GW, Maccallum DK. Culture and growth characteristics of chondrocytes encapsulated in alginate beads. Connect Tissue Res 1989;19:277-297.
30. Loty S, Sautier JM, Loty C, Boulekbache H, Kokubo T, Forest N. Cartilage formation by fetal rat chondrocytes cultured in alginate beads: a proposed model for investigating tissue-biomaterial interactions. J Biomed Mater Res 1998;42:213-222.
31. Lindenhayn K, Perka C, Spitzer RS, Heilmann HH, Pommerening K, Menneke J, Sittinger M. Retention of hyaluronic acid in alginate beads: aspects for in vitro cartilage engineering. J Biomed Mater Res 1999;44:149-155.