Cross-species interactions between malaria parasites in humans

Science

Volumn 287, Issue 5454, 2000, Pages 845-848

  Bruce, Marian C ^a   Donnelly, Christl A ^a   Alpers, Michael P ^a   Galinski, Mary R ^a   Barnwell, John W ^a   Walliker, David ^a   Day, Karen P ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; DISEASE TRANSMISSION; GENOTYPE; HUMAN; IMMUNE RESPONSE; INFECTION RISK; MALARIA; PLASMODIUM; PRIORITY JOURNAL; SPECIES DIFFERENCE;

ADOLESCENT; ANIMALS; CHILD; CHILD, PRESCHOOL; FEMALE; GENOTYPE; HUMANS; MALARIA; MALARIA VACCINES; MALE; PAPUA NEW GUINEA; PARASITEMIA; PLASMODIUM; PLASMODIUM FALCIPARUM; PLASMODIUM MALARIAE; PLASMODIUM VIVAX; SPECIES SPECIFICITY;

PROTOZOA;

EID: 0034602943     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.287.5454.845     Document Type: Article

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21. All samples were analyzed from children with ≥2 P. falciparum smear-positive samples or ≥4 F. vivax positives (15). Blood from finger pricks was collected in EDTA and stored at -70°C, then 20-μl volumes were transferred to filter paper for DNA extraction [S. Kyes, A. G. Craig, K. Marsh, C. I. Newbold, Exp. Parasitol. 77, 473 (1993)]. Plasmodium falciparum genotypes were determined by size and sequence polymorphisms in PCR-amplified alleles of the merozoite surface protein 2 (Msp2) gene [H. Babiker, L. Ranford-Cartwright, A. Sultan, G. Satti, D. Walliker, Trans. R. Soc. Trop. Med. Hyg. 88, 328 (1994)]. Plasmodium vivax genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis [M. C. Bruce, M. R. Galinski, J. W. Barnwell, G. Snouou, K. P. Day, Am. J. Trop. Med. Hyg. 61, 518 (1999)] of alleles at the merozoite surface protein 3 alpha (Msp3α) locus [M. R. Galinski et al., Mol. Biochem. Parasitol. 101, 131 (1999)].
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36. We thank the people of Gonoa for their long-standing cooperation and the staff of the Papua New Guinea Institute of Medical Research, Madang, for their assistance. Supported by grants to K.P.D., D.W., and M.P.A. from the European Commission, by a grant to K.P.D. from the Wellcome Trust, by NIH grant AI24710 to J.W.B., and NIH grant AI37545 and World Health Organization/TDR grants (950440 and 910495) to M.R.G. M.C.B. was supported by The UK Medical Research Council and The Wellcome Trust. C.A.D. was supported by The Wellcome Trust.