Structures of L-fuculose-1-phosphate aldolase mutants outlining motions during catalysis

Journal of Molecular Biology

Volumn 303, Issue 4, 2000, Pages 531-543

  Joerger, Andreas C ^a   Mueller Dieckmann, Christoph ^a   Schulz, Georg E ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

Author keywords

Class II aldolase; Enzyme mechanism; Phosphate binding site; X ray structure analysis; Zinc coordination sphere

Indexed keywords

DNA; FRUCTOSE BISPHOSPHATE ALDOLASE; MUTANT PROTEIN; ZINC;

ARTICLE; BINDING SITE; CATALYSIS; CRYSTAL STRUCTURE; ENZYME ACTIVITY; ENZYME STRUCTURE; GENE MUTATION; PRIORITY JOURNAL; STRUCTURE ANALYSIS; X RAY ANALYSIS;

EID: 0034602369     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.4153     Document Type: Article

References (38)

1. Rabbit muscle aldolase as a catalyst in organic syntheses
2. Product binding and role of the C-terminal region in class I D-fructose 1,6-bisphosphate aldolase
3. Novel active site in Escherichia coli fructose 1,6-bisphosphate aldolase
4. The crystal structure of class II fructose-1,6-bisphosphate aldolase shows a novel binuclear metal-binding active site embedded in a familiar fold
5. Crystal structure of human muscle aldolase complexed with fructose 1,6-bisphosphate: Mechanistic implications
6. The spatial structure of the class II L-fuculose-1-phosphate aldolase from Escherichia coli
7. Refined high-resolution structure of the metal-ion dependent L-fuculose-1-phosphate aldolase (class II) from Escherichia coli
8. Catalytic mechanism of the metal-dependent fuculose aldolase from Escherichia coli as derived from the structure
9. Enzyme-catalyzed aldol additions
10. Diastereoselective enzymatic aldol additions: L-rhamnulose and L-fuculose-1-phosphate aldolases from E. coli
11. 6-Deoxy-L-lyxo- and 6-Deoxy-L-arabino-hexulose 1-phosphates. Enzyme syntheses by antagonistic metabolic pathways
12. The mechanism of class II, metal-dependent aldolases
13. Class I aldolases: Substrate specificity, mechanism, inhibitors and structural aspects
14. The metabolism of L-fucose
15. L-Fuculose 1-phosphate aldolase
16. Calculation of protein extinction coefficients from amino acid sequence data
17. Structure of native and apo carbonic anhydrase II and structure of some of its anion-ligand complexes
18. The crystal structure of Escherichia coli class II fructose-1,6-bisphosphate aldolase in complex with phosphoglycolohydroxamate reveals details of mechanism and specificity
19. Crystal structure and reaction mechanism of 7,8-dihydroneopterin aldolase from Staphylococcus aureus
20. Aldolases
21. Structure-assisted redesign of a protein-zinc-binding site with femtomolar affinity
22. Catalytic action of fuculose 1-phosphate aldolase (class II) as derived from structure-directed mutagenesis
23. Epimerization via carbon-carbon bond cleavage. L-Ribulose-5-phosphate 4-epimerase as a masked class II aldolase
24. Improved methods for building protein models in electron density maps and the location of errors in these models
25. Evaluation of single crystal X-ray diffraction data from a position-sensitive detector
26. Crystal structure of fructose-1,6-bisphosphate aldolase from the human malaria parasite Plasmodium falciparum
27. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures
28. Automated refinement of protein models
29. Structure and mechanism of a sub-family of enzymes related to N-acetylneuraminate lyase
30. Crystal structure of human carbonic anhydrase C
31. Raster3D: Photorealistic molecular graphics
32. Adenylate kinase motions during catalysis: An energetic counterweight balancing substrate binding
33. Refinement of macromolecular structures by the maximum-likelihood method
34. Overproduction and substrate specificity of a bacterial fuculose-1-phosphate aldolase: A new enzymatic catalyst for stereocontrolled aldol condensation
35. The catalytic mechanism of carbonic anhydrase: Implications of a rate-limiting protolysis of water
36. Transitions, transition states, transition state analogues: Zinc pyrazolylborate chemistry related to zinc enzymes
37. Enzymes in organic synthesis: Application to the problems of carbohydrate recognition