Photoregulation of the DNA polymerase reaction by oligunucleotides bearing an azobenzene

Angewandte Chemie - International Edition

Volumn 39, Issue 13, 2000, Pages 2356-2357

  Yamazawa, Akira ^a   Liang, Xingguo ^a   Asanuma, Hiroyuki ^a   Komiyama, Makoto ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

DNA structures; Enzyme catalysis; Photochemistry; Polymerase

Indexed keywords

AZOBENZENE DERIVATIVE; DNA POLYMERASE; FLUORESCEIN ISOTHIOCYANATE;

ARTICLE; CHEMICAL REACTION KINETICS; ENZYME REGULATION; ISOMERISM; PHOTOCHEMISTRY; REACTION ANALYSIS; ULTRAVIOLET IRRADIATION;

EID: 0034600770     PISSN: 14337851     EISSN: None     Source Type: Journal    
DOI: 10.1002/1521-3773(20000703)39:13<2356::AID-ANIE2356>3.0.CO;2-9     Document Type: Article

References (15)

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8. m) of the duplexes between these oligonucleotides and the complementary ones are notably changed on photoirradiation: H. Asanuma, T. Ito, T. Yoshida, X. Liang, M. Komiyama, Angew. Chem. 1999, 111, 2547; Angew. Chem. Int. Ed. 1999, 38, 2393. However, the present photoregulation of the polymerase reaction is based on a different chemistry (see text for details).
9. m) of the duplexes between these oligonucleotides and the complementary ones are notably changed on photoirradiation: H. Asanuma, T. Ito, T. Yoshida, X. Liang, M. Komiyama, Angew. Chem. 1999, 111, 2547; Angew. Chem. Int. Ed. 1999, 38, 2393. However, the present photoregulation of the polymerase reaction is based on a different chemistry (see text for details).
10. When another 54-mer DNA was used as the template, the full-length product was produced for 20 min in almost 100% yield (with respect to the template: see Figure 1 in the Supporting Information). The efficiency of the present photoregulated polymerization considerably depends on the sequences of components. The photoregulation was also successful, when an 89-mer DNA was used as the template.
11. 3-spacer). the dominant product was the 34-mer DNA. even with UV irradiation (see Figure 3 in the Supporting Information).
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13. Consistently, the 34-mer DNA was the dominant product, even under UV irradiation, when the unmodified 20-mer modulator 2a was used in place of 2b.
14. m values (-50 C).
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