Chemically mediated site-specific cleavage of proteins [9]

Journal of the American Chemical Society

Volumn 122, Issue 30, 2000, Pages 7402-7403

  Wang, Bixun ^a   Lodder, Michiel ^a   Zhou, Jia ^a   Baird Jr , Teaster T ^b   Brown, Kathlynn C ^b   Craik, Charles S ^b   Hecht, Sidney M ^a  

^a UNIVERSITY OF VIRGINIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING AFFINITY; BINDING SITE; CATALYSIS; LETTER; PROTEIN BINDING; PROTEIN STRUCTURE; PROTEIN SYNTHESIS;

EID: 0034596319     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja0002262     Document Type: Letter

References (42)

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33. 5f
34. 7a The ability of DHFR analogues to bind to Ni-NTA agarose and methotrexate-agarose was used to determine whether each analogue (i) contained a hexahistidine moiety at the N terminus and (ii) folded in a fashion similar to wild type, respectively.
35. AGly at different positions (Table 1). Wild-type DHFR, and analogues Asp27Val and Glu-1Val gave no cleavage products under the same conditions (Table 1).
36. 2 treatment afforded a protein that comigrated with an authentic standard by high-resolution PAGE and native capillary electrophoresis.
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