Use of transcriptional regulatory sequences of telomerase (hTER and hTERT) for selective killing of cancer cells

Molecular Therapy

Volumn 2, Issue 6, 2000, Pages 539-544

  Abdul Ghani, R ^a   Ohana, P ^a   Matouk, I ^a   Ayesh, S ^a   Ayesh, B ^a   Laster, M ^a   Bibi, O ^a   Giladi, H ^c   Molnar Kimber, K ^b   Sughayer, M A ^d   De Groot, N ^a   Hochberg, A ^a  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

^b HOSPITAL OF THE UNIVERSITY OF PENNSYLVANIA   (United States)

^c HADASSAH HEBREW UNIVERSITY MEDICAL CENTER   (Israel)

^d AL QUDS UNIVERSITY   (Palestine)

Author keywords

Bladder cancer; Diphtheria toxin; Gene therapy; Telomerase

Indexed keywords

DIPHTHERIA TOXIN; DIPHTHERIA TOXIN FRAGMENT A; PEPTIDE FRAGMENT; PRIMER DNA; TELOMERASE;

ARTICLE; BLADDER TUMOR; CELL CULTURE; CELL SURVIVAL; CHEMISTRY; DNA METHYLATION; GENETIC TRANSCRIPTION; GENETICS; HUMAN; LIVER CELL CARCINOMA; LIVER TUMOR; NUCLEOTIDE SEQUENCE; PATHOLOGY; PROMOTER REGION; REGULATORY SEQUENCE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

BASE SEQUENCE; CARCINOMA, HEPATOCELLULAR; CELL SURVIVAL; DIPHTHERIA TOXIN; DNA METHYLATION; DNA PRIMERS; HUMANS; LIVER NEOPLASMS; PEPTIDE FRAGMENTS; PROMOTER REGIONS (GENETICS); REGULATORY SEQUENCES, NUCLEIC ACID; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TELOMERASE; TRANSCRIPTION, GENETIC; TUMOR CELLS, CULTURED; URINARY BLADDER NEOPLASMS;

EID: 0034575796     PISSN: 15250016     EISSN: None     Source Type: Journal    
DOI: 10.1006/mthe.2000.0196     Document Type: Article

References (31)

1. Feng, J., et al. (1995). The RNA component of human telomerase. Science 269: 1236-1241.
2. Nakamura, T. M., et al. (1997). Telomerase catalytic subunit homologs from fission yeast and human. Science 277: 955-959.
3. Cong, Y. S., Wen, J., and Bacchetti S. (1999). The human telomerase catalytic subunit hTERT: Organization of the gene and characterization of the promoter. Hum. Mol. Genet. 8: 137-142.
4. Takakura, et al. (1999). Cloning of human telomerase catalytic subunit (hTERT) gene promoter and identification of proximal core promoter sequences essential for transcriptional activation in immortalized and cancer cells. Clin. Cancer Res. 59: 551-557.
5. Soder, A. I., Hoare, S. F., Muir, S., Going, J. J., Parkinson, E. K., and Keith, W. N. (1997). Amplification, increased dosage and in situ expression of the telomerase RNA gene in human cancer. Oncogene 14: 1013-1021
6. Ito, H., Kyo, S., Kanaya, T., Takakura, M., Inoue, M., and Namiki, M. (1998). Expression of human telomerase subunits and correlation with telomerase activity in urothelial cancer. Clin. Cancer Res. 4: 1603-1608.
7. Takakura, M., Kyo, S., Kanaya, T., Tanaka, M., and Inoue, M. (1998). Expression of human telomerase subunits and correlation with telomerase activity in cervical cancer. Cancer Res. 58: 1558-1561.
8. Kent, S. (1995). Telomerase: The immortalizing enzyme. Update on Geron Corporation. Life Extension Rep. 15.
9. Zhao, J. Q., Hoare, S. F., McFarlane, R., Muir, S., Parkinson, E. K., Black, D. M., and Keith, W. N. (1998). Cloning and characterization of human and mouse telomerase RNA gene promoter sequences. Oncogene 16: 1345-1350.
10. Devereux, T., R., Horikawa, I., Anna, C. H., Annab, L. A., Afshari, C. A., and Barrett, J. C. (1999). DNA methylation analysis of the promoter region of the human telomerase reverse transcriptase (hTERT) gene. Cancer Res. 59: 6087-6090.
11. Sandvig, K., and van Deurs, B. (1992). Toxin-induced cell lysis: protection by 3-methyladenine and cycloheximide. Exp. Cell Res. 200: 253-262.
12. Kochi, S. K, and Collier, R. J. (1993). DNA fragmentation and cytolysis in U937 cells treated with diphtheria toxin or other inhibitors of protein synthesis. Exp. Cell Res. 208: 296-302.
13. Yamaizumi, M., Mekada, E., Uchida, T., and Okada, Y. (1978). One molecule of diphtheria toxin fragment A introduced into a cell can kill the cell. Cell 15: 245-250.
14. Palmiter, R. D., Behringer, R. R., Quaife, C. J., Maxwell, F., Maxwell, I. H., and Brinster, R. L. (1987). Cell lineage ablation in transgenic mice by cell-specific expression of a toxin gene. Cell 50: 435-443.
15. Breitman, M. L., et al. (1987). Genetic ablation: Targeted expression of a toxin gene causes microphthalmia in transgenic genes. Science 238: 1563-1565.
16. Kopf, E., et al. (1998). The effect of retinoic acid on the activation of the human H19 promoter by a 3′downstream region. FEBS Lett. 432: 123-127.
17. Ariel, I., et al. (1998). Imprinted H19 oncofetal RNA is a candidate tumor marker for hepatocellular carcinoma. J. Clin. Pathol. Mol. Pathol. 51: 21-25.
18. Heine, B., Hummel, M., Demel, G., and Stein, H. (1998). Demonstration of constant up regulation of the telomerase RNA component in human gastric carcinomas using in situ hybridization. J. Pathol. 185: 139-144.
19. Kyo, S., Kunimi, K., Uchibayashi, T., Namiki, M., and Inoue, M. (1997). Telomerase activity in human urothelial tumors. Am. J. Clin. Pathol. 5: 555-560.
20. Vaziri, H, and Benchimol, S. (1998). Reconstitution of telomerase activity in normal human cell leads to elongation of telomeres and extended replicative life span. Curr. Biol. 8: 279-282.
21. Bryan, T. M., Englezou, A., Dalla-Pozza, L., Dunham, M. A., and Reddel, R. R. (1997). Evidence for an alternative mechanism for maintaining telomere length in human tumors and tumor-derived cell lines. Nat. Med. 3: 1271-1274.
22. Ito, H., Kyo, S., Kanaya, T., Takakura, M., Inoue, M., and Namiki, M. (1998). Expression of human telomerase subunits and correlation with telomerase activity in urothelial cancer. Clin. Cancer Res. 4: 1603-1608.
23. Nakayama, J., et al. (1998). Telomerase activation by hTERT in human normal fibroblast and hepatocellular carcinomas. Nat. Genet. 18: 65-68.
24. Collins, K. (1996). Structure and function of telomerase. Curr. Opin. Cell Biol. 8: 374-380.
25. Horikawa, I., Cable, P. L., Afshari, C., and Barrett, J. C. (1999). Cloning and characterization of the promoter region of human telomerase reverse transcriptase gene. Cancer Res. 59: 826-830.
26. Maxwell, F., Maxwell, I. H., and Glode, L. M. (1987). Cloning, sequence determination, and expression in transfected cells of the coding sequence for the tox 176 attenuated diphtheria toxin A chain. Mol. Cell. Biol. 7: 1576-1579.
27. Maxwell, I. H., Maxwell, F., and Glode, L. M. (1986). Regulated expression of a diphtheria toxin A-chain gene transfected into human cells: Possible strategy for inducing cancer cell suicide. Cancer Res. 46: 4660-4664.
28. Lidor, Y. J., et al. (1997). In vitro expression of the diphtheria toxin A-chain gene under the control of human chorionic gonadotropin gene promoters as a means of directing toxicity to ovarian cancer cell lines. Am. J. Obstet. Gynecol. 177: 579-585.
29. Massuda, E. S., et al. (1997). Regulated expression of the diphtheria toxin A chain by a tumor-specific chimeric transcription factor results in selective toxicity for alveolar rhabdomyosarcoma cells. Proc. Natl. Acad. Sci. USA 494: 14701-14706.
30. Fisher, K. S., Maxwell, I. H., Murphy, J. R., Collier, J., and Glode, L. M. (1991). Construction and expression of plasmids containing mutated diphtheria toxin A-chain-coding sequences. Infect. Immun. 59: 3562-3565.
31. Goldberg, M. R., et al. (1995). Phase I clinical study of the recombinant oncotoxin TP40 in superficial bladder cancer. Clin. Cancer Res. 1: 57-61.