Genetic Evaluation of Peroxisomal and Cytosolic Acetoacetyl-CoA Thiolase Isozymes in n-Alkane-Assimilating Diploid Yeast, Candida tropicalis

Cell Biochemistry and Biophysics

Volumn 32, Issue , 2000, Pages 285-290

  Ueda, Mitsuyoshi ^a   Kanayama, Naoki ^a   Tanaka, Atsuo ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

3 ketoacyl CoA thiolase; Acetoacetyl CoA thiolase; Candida tropicalis; Cytosol; Mevalonate; Peroxisome

Indexed keywords

ACETYL COENZYME A ACETYLTRANSFERASE; ALKANE; ISOENZYME;

ARTICLE; CANDIDA; CYTOSOL; ENZYMOLOGY; GENETICS; METABOLISM; MUTATION; PEROXISOME; ULTRASTRUCTURE;

ACETYL-COA C-ACETYLTRANSFERASE; ALKANES; CANDIDA; CYTOSOL; ISOENZYMES; MUTATION; PEROXISOMES;

EID: 0034571517     PISSN: 10859195     EISSN: None     Source Type: Journal    
DOI: 10.1385/CBB:32:1-3:285     Document Type: Article

References (15)

1. Kurihara, T., Ueda, M., and Tanaka, A. (1988) Occurrence and possible roles of acetoacetyl-CoA thiolase and 3-ketoacyl-CoA thiolase in peroxisomes of an n-alkane-grown yeast, Candida tropicalis. FEBS Lett. 229, 215-218.
2. Kurihara, T., Ueda, M., and Tanaka, A. (1989) Peroxisomal acetoacetyl-CoA thiolase and 3-ketoacyl-CoA thiolase from an n-alkane-utilizing yeast, Candida tropicalis: purification and characterization. J. Biochem. 106, 474-478.
3. Kurihara, T., Ueda, M., Okada, H., Kamasawa, N., Naito, N., Osumi, M., and Tanaka, A. (1992) β-Oxidation of butyrate, the short-chain-length fatty acid, occurs in peroxisomes in the yeast Candida tropicalis. J. Biochem. 111, 783-787.
4. Kanayama, N., Ueda, M., Atomi, H., Kurihara, T., Kondo, J., Teranishi, Y., and Tanaka, A. (1994) Comparison of molecular structures and regulation of biosynthesis of unique thiolase isozymes localized only in peroxisomes of n-alkane-utilizing yeast, Candida tropicalis. J. Ferment. Bioeng. 78, 273-278.
5. Kurihara, T., Ueda, M., Kanayama, N., Kondo, J., Teranishi, Y., and Tanaka, A. (1992) Peroxisomal acetoacetyl-CoA thiolase of an n-alkane-utilizing yeast, Candida tropicalis. Eur. J. Biochem. 210, 999-1005.
6. Kanayama, N., Himeda, Y., Atomi, H., Ueda, M., and Tanaka, A. (1997) Expression of acetoacetyl-CoA thiolase isozyme genes of n-alkane-assimilating yeast, Candida tropicalis: isozymes in two intracellular compartments are derived from the same genes. J. Biochem. 122, 616-621.
7. Haas, L. O. C., Cregg, J. M., and Gleeson, M. A. G. (1990) Development of an integrative DNA transformation system for the yeast Candida tropicalis. J. Bacteriol. 172, 4571-4577.
8. Kanayama, N., Ueda, M., Atomi, H., and Tanaka, A. (1998) Genetic evaluation of physiological functions of thiolase isozymes in the n-alkane-assimilating yeast Candida tropicalis. J. Bacteriol. 180, 690-698.
9. Burgers, P. M. J. and Percival, K. J. (1987) Transformation of yeast spheroplasts without cell fusion. Anal. Biochem. 163, 391-397.
10. Gould, S. J., Keller, G. A., Hosken, N., Wilkinson, J., and Subramani, S. (1989) A conserved tripeptide sorts proteins to peroxisomes. J. Cell Biol. 108, 1657-1664.
11. Thompson, S. L. and Krisans, S. K. (1990) Rat liver peroxisomes catalyze the initial step in cholesterol synthesis. The condensation of acetyl-CoA units into acetoacetyl-CoA. J. Biol. Chem. 265, 5731-5735.
12. Keller, G. A., Pazirandeh, M., and Krisans, S. (1986) 3-Hydroxy-3-methylglutaryl coenzyme A reductase localization in rat liver peroxisomes and microsomes of control and cholestyramine-treated animals: quantitative biochemical and immunoelectron microscopical analyses. J. Cell Biol. 103, 875-886.
13. Kurihara, T., Ueda, M., Okada, H., Kamasawa, N., Osumi, M., and Tanaka, A. (1992) Physiological roles of acetoacetyl-CoA thiolase in n-alkane-utilizable yeast, Candida tropicalis: possible contribution to alkane degradation and sterol biosynthesis. J. Biochem. 112, 845-848.
14. Danpure, C. J. (1995) How can the products of a single gene be localized to more than one intracellular compartment? Trends Cell Biol. 5, 230-238.
15. Purdue, P. E. and Lazarow, P. B. (1996) Targeting of human catalase to peroxisomes is dependent upon a novel COOH-terminal peroxisomal targeting sequence. J. Cell Biol. 134, 849-862.