Purification of GroEL from an Overproducing E. coliStrain

Methods in Molecular Biology

Volumn 140, Issue , 2000, Pages 29-39

  Quaite Randall, Elsie ^a   Joachimiak, Andrzej ^a  

^a ARGONNE NATIONAL LABORATORY   (United States)

Author keywords

Apical Domain; Centrifugal Concentrator; GroE Operon; Native Page; Plastic Backing

Indexed keywords

BUFFER; CHAPERONIN; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS;

ARTICLE; CHEMISTRY; ESCHERICHIA COLI; GEL CHROMATOGRAPHY; GENETICS; GROWTH, DEVELOPMENT AND AGING; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METHODOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; TWO DIMENSIONAL GEL ELECTROPHORESIS;

BUFFERS; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GROEL PROTEIN; INDICATORS AND REAGENTS;

MLCS; MLOWN;

EID: 0034570811     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1385/1-59259-061-6:29     Document Type: Chapter

References (7)

1. Hemmingsen, S. M., Woodford, C., van der Vies, S., Tilly, T., Dennis, D. T., Georgopolus, C. P., Hendrix, R. W., and Ellis, R. J. (1988) Homologous plant and bacterial proteins chaperone oligomeric protein assembly Nature 333, 330–334.
2. Braig, K., Otwinowski, Z., Hedge, R., Boisvert, D. C., Joachimiak, A., Horwich, A. L., and Sigler, P. B. (1994) The crystal structure of the bacterial chaperonin GroEL at 2.8 A. Nature, 371, 578–586.
3. Lorimer, G. and Baldwin, T. (eds) (1998) Molecular Chaperones, in Methods in Enzymology,vol. 290, Academic.
4. Lammeli, U. K. (1970) Clevage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.
5. Quaite-Randall, E., Trent, J. D., Josephs, R., and Joachimiak, A. (1995) Conformational cycle of the Archaeosome, a TCP-1-like chaperonin from Sulfolobus shibatae. J. Biol. Chem. 270, 28818.
6. Giometti, C. S. Gemmel, M. A. Tollaksen, S. L., and Taylor, J., (1991) Quantitation of human leukocyte proteins after silver staining: A study with two dimensional electrophoresis. Electrophoresis 12, 536–543.
7. Gill, S. C. and von Hipple P. H. (1989) Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 182, 319–326.