GroEL/GroES Interaction Assayed by Protease Protection

Methods in Molecular Biology

Volumn 140, Issue , 2000, Pages 71-74

  Martin, Jörg ^a  

^a BROWN UNIVERSITY   (United States)

Author keywords

Cylinder Cavity; Flexible Tail; GroES Protein; Propanesulfonic Acid; Protease Protection

Indexed keywords

CHAPERONIN; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; PROTEINASE K;

ARTICLE; CHEMISTRY; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR WEIGHT; POLYACRYLAMIDE GEL ELECTROPHORESIS;

ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOPEPTIDASE K; GROEL PROTEIN; GROES PROTEIN; INDICATORS AND REAGENTS; MOLECULAR WEIGHT;

MLCS; MLOWN;

EID: 0034570491     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1385/1-59259-061-6:71     Document Type: Chapter

References (4)

1. Langer, T., Pfeifer, G., Martin, J., Baumeister, W., and Hartl, F.-U. (1992) Chaperonin-mediated protein folding: GroES binds to one end of the GroEL cylinder, which accommodates the protein substrate within its central cavity. EMBO J. 11, 4757–4765.
2. Martin, J., Mayhew, M., Langer, T., and Hartl, F. U. (1993) The reaction cycle of GroEL and GroES in chaperonin-assisted protein folding. Nature 366, 228–233.
3. Braig, K., Otwinowski, Z., Hegde, R., Boisvert, D. C., Joachimiak, A., Horwich, A. L., and Sigler, P. B. (1994) The crystal structure of the bacterial chaperonin GroEL at 2.8 A. Nature 371, 578–586.
4. Hayer-Hartl, M. K., Martin, J., and Hartl, F. U. (1995) Asymmetrical interaction of GroEL and GroES in the ATPase cycle of assisted protein folding. Science 269, 836–841.