Divergent enzyme kinetics and structural properties of the two human mitochondrial creatine kinase isoenzymes

Biological Chemistry

Volumn 381, Issue 11, 2000, Pages 1063-1070

  Schlattner, U ^a   Eder, M ^a   Dolder, M ^a   Khuchua, Z A ^a   Strauss, A W ^a   William, T ^a  

^a ETH ZURICH   (Switzerland)

Author keywords

2D protein crystals; Energy metabolism; Enzyme kinetics; Guanidino kinase; Protein purification

Indexed keywords

ADENOSINE TRIPHOSPHATE; CARDIOLIPIN; CREATINE; CREATINE KINASE ISOENZYME; DIMER; MITOCHONDRIAL ENZYME; PHOSPHOLIPID;

ARTICLE; BIOCHEMISTRY; CELL ENERGY; CHEMICAL REACTION; CONFORMATION; CONTROLLED STUDY; ENERGY METABOLISM; ENZYME BINDING; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME STABILITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; HEALTH; HUMAN; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATION; PHYSICAL DISEASE; PRIORITY JOURNAL; PROTEIN EXPRESSION; SARCOMERE;

CREATINE KINASE; CREATINE KINASE, MITOCHONDRIAL FORM; GENE EXPRESSION; HUMANS; ISOENZYMES; KINETICS; MICROSCOPY, ELECTRON; RECOMBINANT FUSION PROTEINS;

EID: 0034536628     PISSN: 14316730     EISSN: None     Source Type: Journal    
DOI: 10.1515/BC.2000.131     Document Type: Article

References (48)

1. Mitochondrial dysfunction in neurodegenerative diseases
2. Complexes between porin, hexokinase, mitochondrial creatine kinase and adenylate translocator display properties of the permeability transition pore. Implication for regulation of permeability transition by the kinases
3. Mitochondrial creatine kinase from human heart muscle: Purification and characterization of the crystallized isoenzyme
4. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
5. Statistical analysis of enzyme kinetic data
6. The mitochondrial permeability transition pore and its role in cell death
7. Crystal structure of human ubiquitous mitochondrial creatine kinase
8. The internal structure of mitochondria
9. Structure of mitochondrial creatine kinase
10. Expression of active octameric chicken cardiac mitochondrial creatine kinase in Escherichia coli
11. Creatine kinase: The reactive cysteine is required for synergism but is nonessential for catalysis
12. Purification and characterization of human mitochondrial creatine kinase. A single enzyme form
13. Kinetics of assembly and dissociation of the mitochondrial creatine kinase octamer. A fluorescence study
14. Isolation and characterization of the gene and cDNA encoding human mitochondrial creatine kinase
15. Separate nuclear genes encode sarcomere-specific and ubiquitous human mitochondrial creatine kinase isoenzymes
16. Mitochondrial creatine kinase as a tumor-associated marker
17. Alterations in the creatine kinase system in the myocardium of cardiomyopathic hamsters
18. Octamer formation and coupling of cardiac sarcomeric mitochondrial creatine kinase are mediated by charged N-terminal residues
19. Expression of creatine kinase isozymes in Hodgkin's derived cell lines
20. Expression of functional mitochondrial creatine kinase in liver of transgenic mice
21. Inhibition of adenosine 5'-triphosphate-creatine phosphotransferase by substrateanion complexes. Evidence for the transition-state organization of the catalytic site
22. The role of creatine kinase in inhibition of mitochondrial permeability transition
23. Crystalline mitochondrial inclusion bodies isolated from creatine depleted rat soleus muscle
24. Expression of the mitochondrial creatine kinase genes
25. Mitochondrial creatine kinase in cancer patients
26. Mitochondrial creatine kinase mediates contact formation between mitochondrial membranes
27. Plotting methods for analyzing enzyme rate data
28. DNA sequencing with chain-terminating inhibitors
29. Functional aspects of the X-ray structure of mitochondrial creatine kinase: A molecular physiology approach
30. Octamers of mitochondrial creatine kinase isoenzymes differ in stability and membrane binding
31. A quantitative approach to membrane binding of human ubiquitous mitochondrial creatine kinase using surface plasmon resonance
32. Native mitochondrial creatine kinase forms octameric structures. II. Characterization of dimers and octamers by ultracentrifugation, direct mass measurements by scanning transmission electron microscopy, and image single mitochondrial creatine kinase
33. Crystallization of mitochondrial creatine kinase on negatively charged lipid layers
34. Octamer-dimer transitions of mitochondrial creatine kinase in heart disease
35. Mitochondrial creatine kinase is a prime target of peroxynitrite-induced modification and inactivation
36. Oligomeric state and membrane binding behaviour of creatine kinase isoenzymes: Implications for cellular function and mitochondrial structure
37. Use of T7 RNA polymerase to direct expression of cloned genes
38. Relative phosphocreatine and nucleoside triphosphate concentrations in cerebral gray and white matter measured in vivo by 31P nuclear magnetic resonance
39. Localization of creatine kinase isoenzymes in myofibrils. I. Chicken skeletal muscle
40. Function of M-line-bound creatine kinase as intramyofibrillar ATP regenerator at the receiving end of the phosphorylcreatine shuttle in muscle
41. Intracellular compartmentation, structure and function of creatine kinase isoenzymes in tissues with high and fluctuating energy demands: The 'phosphocreatine circuit' for cellular energy homeostasis
42. Purification and characterization of human sarcomeric mitochondrial creatine kinase
43. Mitochondrial creatine kinase (EC 2.7.3.2) in the brain
44. Mitochondrial creatine kinase: A key enzyme of aerobic energy metabolism
45. Limited proteolysis of creatine kinase. Implications for three-dimensional structure and for conformational substrates
46. Creatine and creatinine metabolism