Characterization of active-site mutants of Schizosaccharomyces pombe phosphoglycerate mutase: Elucidation of the roles of amino acids involved in substrate binding and catalysis

European Journal of Biochemistry

Volumn 267, Issue 24, 2000, Pages 7065-7074

  Nairn, Jacqueline ^a   Duncan, Doris ^a   Price, Naomi E ^a   Kelly, Sharon M ^a   Fothergill Gilmore, Linda A ^b   Uhrinova, Stanislava ^b   Barlow, Paul N ^b   Rigden, Daniel J ^c   Price, Nicholas C ^a,d  

^a UNIVERSITY OF STIRLING   (United Kingdom)

^b UNIVERSITY OF EDINBURGH   (United Kingdom)

^c UNIVERSIDADE CATÓLICA DE BRASÍLIA   (Brazil)

^d UNIVERSITY OF GLASGOW   (United Kingdom)

Author keywords

Catalytic mechanism; Phosphoglycerate mutase; Protein stability; Site directed mutagenesis; Substrate binding

Indexed keywords

3 PHOSPHOGLYCERIC ACID; AMINO ACID; GLYCERIC ACID; GUANIDINE; PHOSPHOGLYCERATE MUTASE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CALORIMETRY; CATALYSIS; CIRCULAR DICHROISM; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME BINDING; ENZYME DENATURATION; ENZYME KINETICS; ENZYME MECHANISM; ENZYME PHOSPHORYLATION; ENZYME STABILITY; ENZYME STRUCTURE; FLUORESCENCE SPECTROSCOPY; GENE EXPRESSION SYSTEM; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES POMBE; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; AMINO ACIDS; BASE SEQUENCE; BINDING SITES; CATALYTIC DOMAIN; DNA PRIMERS; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PHOSPHOGLYCERATE MUTASE; PROTEIN FOLDING; SCHIZOSACCHAROMYCES; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

EID: 0034536210     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2000.01802.x     Document Type: Article

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