Role of arginine 177 in the MnII binding site of manganese peroxidase: Studies with R177D, R177E, R177N, and R177Q mutants

European Journal of Biochemistry

Volumn 267, Issue 24, 2000, Pages 7038-7045

  Sollewijn Gelpke, Maarten D ^a   Youngs, Heather L ^a   Gold, Michael H ^a  

^a OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

Author keywords

Hydrogen peroxide; Lignin degrading enzymes; Manganese; Mn peroxidase; Mutants

Indexed keywords

HYDROGEN PEROXIDE; LIGNIN; MANGANESE; MANGANESE PEROXIDASE;

AMINO ACID SEQUENCE; ARTICLE; BINDING SITE; DISSOCIATION CONSTANT; ELECTRON TRANSPORT; ENZYME BINDING; ENZYME MECHANISM; ENZYME SUBSTRATE COMPLEX; MICHAELIS CONSTANT; NONHUMAN; OXIDATION REDUCTION REACTION; PHANEROCHAETE; PRIORITY JOURNAL;

ARGININE; BINDING SITES; KINETICS; MANGANESE; PEROXIDASES; PHANEROCHAETE; RECOMBINANT PROTEINS;

EID: 0034535788     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2000.01798.x     Document Type: Article

References (50)

1. Lignin biodegradation
2. Enzymatic 'combustion': The microbial degradation of lignin
3. Extracellular peroxidases involved in lignin degradation by the white rot basidiomycete Phanerochaete chrysosporium
4. Biodegradation of DDT [1,1,1-trichloro-2,2-bis (4-chlorophenyl) ethane] by the white rot fungus Phanerochaete chrysosporium
5. Organopollutant degradation by ligninolytic fungi
6. Degradation of 2,4,5-trichlorophenol by the lignin-degrading basidiomycete Phanerochaete chrysosporium
7. Degradation of 2,4,6-trichlorophenol by Phanerochaete chrysosporium: Involvement of reductive dechlorination
8. Molecular biology of the lignindegrading basidiomycete Phanerochaete chrysosporium
9. Ligninolysis by a purified lignin peroxidase
10. In vitro depolymerization of lignin by manganese peroxidase of Phanerochaete chrysosporium
11. Oxidative degradation of non-phenolic lignin during lipid peroxidation by fungal manganese peroxidase
12. Biodegradation mechanism of lignin by white-rot basidiomycetes
13. Oxidation of dimethoxylated aromatic compounds by lignin peroxidase from Phanerochaete chrysosporium
14. New mechanism of the Cα-Cβ cleavage in non-phenolic arylglycerol β-aryl ether lignin substructures catalyzed by lignin peroxidase
15. Mechanism of β-aryl ether dimeric lignin model compound oxidation by lignin peroxidase of Phanerochaete chrysosporium
16. Oxidation of phenolic arylglycerol β-aryl ether lignin model compounds by manganese peroxidase from Phanerochaete chrysosporium: Oxidative cleavage of an α-carbonyl model compound
17. Mechanism of oxidative Cα-Cβ cleavage of a lignin model dimer by Phanerochaete chrysosporium ligninase. Stoichiometry and involvement of free radicals
18. Manganese peroxidase
19. Purification and characterization of an extracellular Mn (II)-dependent peroxidase from the lignin-degrading basidiomycete, Phanerochaete chrysosporium
20. Mn (II) oxidation is the principal function of the extracellular Mn-peroxidase from Phanerochaete chrysosporium
21. The crystal structure of manganese peroxidase from Phanerochaete chrysosporium at 2.06-Å resolution
22. Spectral characterization of manganese peroxidase, an extracellular heme enzyme from the lignin-degrading basidiomycete, Phanerochaete chrysosporium
23. IH NMR investigation of manganese peroxidase from Phanerochaete chrysosporium. A comparison with other peroxidases
24. On the function and mechanism of action of peroxidase
25. Spectral characterization of the oxidized states of lignin peroxidase, an extracellular heme enzyme from the white-rot basidiomycete Phanerochaete chrysosporium
26. Manganese peroxidase from the basidiomycete Phanerochaete chrysosporium: Spectral characterization of the oxidized states and the catalytic cycle
27. Manganese (II) oxidation by manganese peroxidase from the basidiomycete Phanerochaete chrysosporium. Kinetic mechanism and role of chelators
28. Mechanism of manganese peroxidase compound II reduction: Effect of organic acid chelators and pH
29. Kinetic analysis of manganese peroxidase. The reaction with manganese complexes
30. Thiol-mediated oxidation of nonphenolic lignin model compounds by manganese peroxidase of Phanerochaete chrysosporium
31. Manganese-dependent peroxidase from Phanerochaete chrysosporium: Primary structure deduced from cDNA sequence
32. Characterization of a cDNA encoding a manganese peroxidase, from the lignin-degrading basidiomycete Phanerochaete chrysosporium
33. Characterization of the gene encoding manganese peroxidase isozyme 3 from Phanerochaete chrysosporium
34. Crystallization and X-ray diffraction analysis of recombinant Coprinus cinereus peroxidase
35. The crystal structure of peanut peroxidase
36. Crystal structure of lignin peroxidase
37. Superfamily of plant, fungal and bacterial peroxidases
38. The manganese binding site of manganese peroxidase: Characterization of an Asp 179 Asn site-directed mutant protein
39. Characterization of manganese (II) binding site mutants of manganese peroxidase
40. 2+ by both compound I and compound II
41. Arginine 177 is involved in MnlI binding by manganese peroxidase
42. Crystal structures of substrate binding site mutants of manganese peroxidase
43. Transformation of Phanerochaete chrysosporium and Neurospora crassa with adenine biosynthetic genes from Schizophyllum commune
44. A reporter gene construct for studying the regulation of manganese peroxidase gene expression
45. Homologous expression of recombinant manganese peroxidase in Phanerochaete chrysosporium
46. Mating system and basidiospore formation in the lignin-degrading basidiomycete Phanerochaete chrysosporium
47. Manganese peroxidase from the lignin-degrading basidiomycete Phanerochaete chrysosporium. Transient state kinetics and reaction mechanism
48. II oxidation and thermal stabilization of the enzyme
49. Prostaglandin H synthase and hydroperoxides: Peroxidase reaction and inactivation kinetics
50. Haloperoxidase activity of manganese peroxidase from. Phanerochaete Chrysosporium