The hyper-thermostable Fe-superoxide dismutase from the archaeon Acidianus ambivalens: Characterization, recombinant expression, crystallization and effects of metal exchange

Biological Chemistry

Volumn 381, Issue 11, 2000, Pages 1089-1101

  Kardinahl, S ^a   Anemuller, S ^a   Schafer, G ^a  

^a UNIVERSITY OF LÜBECK   (Germany)

Author keywords

Archaea; Hyperthermophiles; Metal substitution; Metallo proteins; Superoxide dismutase; Thermostability

Indexed keywords

COBALT; COPPER; IRON; MANGANESE; NICKEL; RECOMBINANT ENZYME; SUPEROXIDE DISMUTASE;

ACIDIANUS AMBIVALENS; ANAEROBIC BACTERIUM; ARCHAEBACTERIUM; ARTICLE; ATOMIC ABSORPTION SPECTROMETRY; CATALYSIS; CONFORMATION; CRYSTALLIZATION; DIFFERENTIAL SCANNING CALORIMETRY; ELECTRON SPIN RESONANCE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ESCHERICHIA COLI; GENE SEQUENCE; HEAT TOLERANCE; MEASUREMENT; MELTING POINT; MOLECULAR CLONING; MOLECULAR INTERACTION; MOLECULAR STABILITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; THERMOSTABILITY; ULTRAVIOLET SPECTROSCOPY;

AMINO ACID SEQUENCE; CLONING, MOLECULAR; COPPER; CRYSTALLIZATION; ELECTRON SPIN RESONANCE SPECTROSCOPY; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; GENES, ARCHAEAL; IRON; MANGANESE; METALS; MOLECULAR SEQUENCE DATA; RECOMBINANT FUSION PROTEINS; SPECTROPHOTOMETRY, ULTRAVIOLET; SULFOLOBACEAE; SUPEROXIDE DISMUTASE;

EID: 0034535450     PISSN: 14316730     EISSN: None     Source Type: Journal    
DOI: 10.1515/BC.2000.134     Document Type: Article

References (56)

1. The primary structure of superoxide dismutase purified from anaerobically maintained Bacteroides gingivalis
2. A cytochrome aa3-type quinol oxidase from Desulfurolobus ambivalens, the most acidophilic archaeon
3. Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels
4. Escherichia coli expresses a copper-and zinc-containing superoxide dismutase
5. Conserved patterns in superoxide dismutase structure
6. The structure of human mitochondrial manganese superoxide dismutase reveals a novel tetrameric interface of two 4-helix bundles
7. X-ray structure analysis of the iron-dependent superoxide dismutase from Mycobacterium tuberculosis at 2.0 Ångstroms resolution reveals novel dimer-dimer interactions
8. Iron superoxide dismutase from the archaeon Sulfolobus solfataricus: Average hydrophobicity and amino acid weight are involved in the adaptation of proteins to extreme environments
9. 16s rDNA-based phylogeny of the archaeal order Sulfolobales and reclassification of Desulfurolobus ambivalens as Acidianus ambivalens comb. nov
10. Metal uptake of recombinant cambialistic superoxide dismutase from Propionibacterium shermanii is affected by growth conditions of host Escherichia coli cells
11. Evolution of Cu, Zn superoxide dismutases and the greek-key beta-barrel structural motive
12. Characterization of the O2-induced manganese-containing superoxide dismutase from Bacteroides fragilis
13. A rapid boiling method for the isolation of bacterial plasmids
14. High efficiency transformation of Escherichia coli with plasmids
15. Isolation, characterization and crystallization of an iron superoxide dismutase from the crenarchaeon Sulfolobus acidocaldarius
16. Electron paramagnetic resonance spectra of some active cobalt(II) substituted metalloenzymes and other cobalt(II) complexes
17. Differential expression of superoxide dismutase containing Ni and Fe/Zn in Streptomyces coelicolor
18. Isolation and characterization of the iron-containing superoxide dismutase of Methanobacterium bryantii
19. Molecular characterization of a DNA ligase gene of the extremely thermophilic archaeon Desulfurolobus ambivalens shows close phylogenetic relationship to eukaryotic ligases
20. Molecular characterization of the sor gene, which encodes the sulfur oxygenase/reductase of the thermoacidophilic archaeon Desulfurolobus (Acidianus) ambivalens
21. Refined crystal structure of superoxid dismutase from the hyperthermophilic archaeon Sulfolobus acidocaldarius at 2.2 A resolution
22. Copper-zinc superoxidase dismutases of Haemophilus influenzae and H. parainfluenzae
23. The crystal structure of an Fe-Superoxide dismutase from the hyperthermophile Aquifex pyrophilus at 1.9 A resolution: Structural basis for thermostability
24. Manganese superoxid dismutase from Thermus thermophilus: A structural model refined at 1.8 Angstroem resolution
25. A Streptococcus mutans superoxide dismutase that is active with either manganese or iron as a cofactor
26. Iron- and manganese-containing superoxide dismutase from Methylomonas J: Identity of the protein moiety and amino acid sequence
27. A pulse-radiolysis study of the catalytic mechanism of the manganese-containing superoxide dismutase from Bacillus stearothermophilus. A kinetic model for the enzyme action
28. Synthesis of either Fe- or Mn-superoxide dismutase with an apparently identical protein moiety by an anaerobic bacterium dependent on the metal supplied
29. In vivo incorporation of cobalt into Propionibacterium shermanii superoxide dismutase
30. The signal recognition particle receptor alpha subunit of the hyperthermophilic archaeon Acidianus ambivalens exhibits an intrinsic GTP hydrolyzing activity
31. Crystal structure of manganese superoxide dismutase from Bacillus stearothermophilus at 2.4 A resolution
32. Isolation and reconstitution of iron-and manganese-containing superoxide dismutase from Bacteroides theataiotaomicron
33. An alternative to digoxigenin-labeled primers for manual nonreadioactive sequencing allows reading of more than 700 bases
34. The terminal quinol oxidase of the hyperthermophilic archaeon Acidianus ambivalens exhibits a novel subunit structure and gene organization
35. Three extremely thermostable proteins from Sulfolobus and a reappraisal of the traffic rules
36. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the Range from 1 to 100 KDa
37. Purification and characterization of the Rieske iron-sulfur protein from the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius
38. Expression of the Sulfolobus acidocaldarius Rieske iron sulfur protein II (SoxF) with the correctly inserted [2Fe2S] cluster in Escherichia coli
39. X-ray structure of the cambialistic superoxide dismutase from Propionibacterium shermanii active with Fe or Mn
40. Superoxide dismutase from the archaebacterium Thermoplasma acidophilum
41. Regulation of an in vivo metal-exchangeable superoxide dismutase from Propionibacterium shermanii exhibiting activity with different metal cofactors
42. Iron superoxide dismutase from Escherichia coli at 3.1-A resolution: A structure unlike that of copper/zinc protein at both monomer and dimer levels
43. Structure-function relationships in iron and manganese superoxide dismutases
44. Bacteriocuprein superoxide dismutases in pseudomonads
45. Copper-zinc superoxide dismutase of Caulobacter crescentus: Cloning, sequencing and mapping of the gene and periplasmic location of the enzyme
46. The 2.1 Angstroem resolution structure of iron superoxid dismutase from pseudomonas ovalis
47. Unique characteristics of a superoxide dismutase of a strictly anaerobic Methanobacterium thermoautotrophicum
48. Regulation and protective role of the microbial superoxidase dismutases
49. Iron superoxide dismutase from the archaeon Sulfolobus solfataricus: Analysis of the structure and thermostability
50. Evidence for axial coordination of 5,6-dimethylbenzimidazole to the cobalt atom of adenosylcobalamin bound to diol dehydratase
51. A novel nickel-containing superoxide dismutase from Streptomyces spp
52. Desulfurolobus ambivalens, gen. nov., Sp. nov., an autotrophic archaebacterium facultatively oxidizing or reducing sulfur