The iron-sulfur clusters in 2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans: Biochemical and spectroscopic investigations

European Journal of Biochemistry

Volumn 267, Issue 24, 2000, Pages 7082-7093

  Hans, Marcus ^a   Buckel, Wolfgang ^a   Bill, Eckhard ^b  

^a PHILIPPS UNIVERSITY MARBURG   (Germany)

^b MAX PLANCK INSTITUTE FOR CHEMICAL ENERGY CONVERSION   (Germany)

Author keywords

2 hydroxyglutaryl CoA dehydratase; EPR spectroscopy; Iron sulfur cluster; Kramers spin system S = 3 2; M ssbauer spectroscopy

Indexed keywords

2 HYDROXYGLUTARYL COENZYME A; 2 HYDROXYGLUTARYL COENZYME A DEHYDRATASE; ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; DITHIONITE; FLAVINE MONONUCLEOTIDE; GLUTACONYL COENZYME A; GLUTAMIC ACID DERIVATIVE; HYDROLYASE; IRON 57; IRON SULFUR PROTEIN; MAGNESIUM CHLORIDE; NITROGENASE; OXYGEN; RIBOFLAVIN; STREPTAVIDIN; TITANIUM DERIVATIVE; UNCLASSIFIED DRUG;

ACIDAMINOCOCCUS FERMENTANS; AFFINITY CHROMATOGRAPHY; ANAEROBIC BACTERIUM; ARTICLE; CONTROLLED STUDY; DEHYDRATION; ELECTRON SPIN RESONANCE; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME STABILITY; ENZYME STRUCTURE; MOSSBAUER SPECTROSCOPY; NONHUMAN; PRIORITY JOURNAL; REDUCTION; STRUCTURE ACTIVITY RELATION; STRUCTURE ANALYSIS; TEMPERATURE; ULTRAVIOLET SPECTROSCOPY;

AMINO ACID SEQUENCE; BACILLUS; BASE SEQUENCE; CHROMATOGRAPHY, AFFINITY; CLOSTRIDIUM; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HYDRO-LYASES; IRON-SULFUR PROTEINS; SPECTRUM ANALYSIS;

EID: 0034535303     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2000.01809.x     Document Type: Article

References (50)

1. Acidaminococcus gen. n., Acidaminococcus fermentans sp. n., anaerobic gram-negative diplococci using amino acids as the sole energy source for growth
2. Two pathways of glutamate fermentation by anaerobic bacteria
3. The reversible dehydration of (R)-2-hydroxyglutarate to (E)-glutaconate
4. Purification of 2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans. An iron-sulfur protein
5. Activation of (R)-2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans
6. Unusual dehydrations in anaerobic bacteria: Considering ketyls (radical anions) as reactive intermediates in enzymatic reactions
7. 2-hydroxyglutaryl-CoA dehydratase from Clostridium symbiosum
8. The phylogeny of the genus clostridium: Proposal of five new genera and eleven species combinations
9. Genes coding for the benzoyl-CoA pathway of anaerobic aromatic metabolism in the bacterium Thauera aromatica
10. A cluster of bacterial genes for anaerobic benzene ring biodegradation
11. Crystallographic structure of the nitrogenase iron protein from Azotobacter vinelandii
12. Biotin-dependent decarboxylases as bacterial sodium pumps: Purification and reconstitution of glutaconyl-CoA decarboxylase from Acidaminococcus fermentans
13. Identification of the gene encoding the activator of (R)-2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans by gene expression in Escherichia coli
14. Simulation of the EMR spectra of high spin iron in proteins
15. A method for the simulation of powder electron spin resonance spectra when S > 1/2
16. Ironcontaining proteins and related analogs - Complementary mossbauer
17. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding
18. 2-Hydroxyglutaryl-CoA dehydratase from Fusobacterium nucleatum (subsp. nucleatum): An iron-sulfur flavoprotein
19. Iron-sulfur clusters and their electronic and magnetic properties
20. On the prosthetic group (s) of component 1I from nitrogenase. EPR of the Fe-protein from Azotobacter vinelandii
21. 1+ cluster with spin S = 3/2
22. EPR and Mossbauer studies of nucleotide-bound nitrogenase iron protein from Azotobacter vinelandii
23. Redox properties and EPR spectroscopy of the P clusters of Azotobacter vinelandii MoFe protein
24. Spectroscopic characterization of the novel iron-sulfur cluster in Pyrococcus furiosus ferredoxin
25. Synthetic analogues of the 4-Fe active sites of reduced ferredoxins. Electronic properties
26. 3. Synthetic analogues of the reduced clusters in ferredoxins and other iron sulfur proteins: Cases of extreme sensitivity
27. Alternative spin states in synthetic analogues of biological clusters: Spin quartet ground states and structures
28. + cluster in the A33Y variant of Pyrococcus furiosus ferredoxin
29. Reactions with the oxidized iron protein of Azotobacter vinelandii nitrogenase: Formation of a 2Fe center
30. -)-stabilized nitrogenase complex and its implications for signal transduction
31. Crystal structure of the cystine C-S lyase from Synechocystis: Stabilization of cysteine persulfide for FeS cluster biosynthesis
32. Cysteine desulfurase activity indicates a role for NifS in metallocluster biosynthesis
33. Magnetic circular dichroism and electron paramagnetic resonance studies of hydrogenases I and II from Clostridium pasteurianum
34. Spectroscopic characterization of the iron-sulfur cluster in Bacillus subtilis glutamine phosphoribosylpyrophosphate amidotransferase
35. Mossbauer study of CO dehydrogenase from Clostridium thermoaceticum
36. 2: [4Fe-4S] to [2Fe-2S] conversion with loss of biological activity
37. An ATPase domain common to prokaryotic cell cycle proteins, sugar kinases, actin, and Hsp70 heat shock proteins
38. Benzoyl-CoA reductase (dearomatizing), a key enzyme of anaerobic aromatic metabolism. A study of adenosinetriphosphatase activity, ATP stoichiometry of the reaction and EPR properties of the enzyme
39. Lactate reduction in Clostridium propionicum. Purification and properties of lactyl-CoA dehydratase
40. (R)-Lactyl-CoA dehydratase from Clostridium propionicum. Stereochemistry of the dehydration of (R)-2-hydroxybutyryl-CoA to crotonyl-CoA
41. Fe-S centers in lactyl-CoA dehydratase
42. Aconitase, a two-faced protein: Enzyme and iron regulatory factor
43. Optical and EPR characterization of different species of active and inactive aconitase
44. Mössbauer and EPR studies of activated aconitase: Development of a localized valence state at a subsite of the [4Fe-4S] cluster on binding of citrate
45. Iron-sulfur cluster-containing L-serine dehydratase from Peptostreptococcus asaccharolyticus: Correlation of the cluster type with enzymatic activity
46. 4-Hydroxybutyryl-CoA dehydratase from Clostridium aminobutyricum: Characterization of FAD and iron-sulfur clusters involved in an overall non-redox reaction