Differential mechanism-based labeling and unequivocal activity assignment of the two active sites of intestinal lactase/phlorizin hydrolase

European Journal of Biochemistry

Volumn 267, Issue 24, 2000, Pages 6996-7005

  Díaz Arribas, Juan C ^a   Herrero, Alicia G ^a   Martín Lomas, Manuel ^b   Javier Canada F ^a   He, Shouming ^c   Withers, Stephen G ^c  

^a INSTITUTO DE QUÍMICA ORGÁNICA GENERAL   (Spain)

^b INSTITUTO DE INVESTIGACIONES QUÍMICAS   (Spain)

^c UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

Active site labeling; Intestinal lactase; Mechanism based inhibitors; Phlorizin hydrolase

Indexed keywords

2',4' DINITROPHENYL 2 DEOXY 2 FLUORO BETA DEXTRO GALACTOPYRANOSIDE; 2',4' DINITROPHENYL 2 DEOXY 2 FLUORO BETA DEXTRO GLUCOPYRANOSIDE; GLYCOSIDASE INHIBITOR; GLYCOSYLCERAMIDASE; LACTASE; LACTOSE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME MECHANISM; LAMB; NONHUMAN; PEPTIDE MAPPING; PRIORITY JOURNAL; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; ANIMALS; BETA-GALACTOSIDASE; BINDING SITES; CARBOHYDRATE SEQUENCE; ENZYME INHIBITORS; GLYCOSYLCERAMIDASE; INTESTINES; LACTASE; MOLECULAR SEQUENCE DATA; PEPTIDE MAPPING; SEQUENCE HOMOLOGY, AMINO ACID; SHEEP;

EID: 0034531969     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2000.01784.x     Document Type: Article

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