Co-expression of the capsid proteins of Cowpea mosaic virus in insect cells leads to the formation of virus-like particles

Journal of General Virology

Volumn 81, Issue 12, 2000, Pages 3093-3097

  Shanks, M ^a   Lomonossoff, G P ^a  

^a JOHN INNES CENTRE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CAPSID PROTEIN; COAT PROTEIN; HYBRID PROTEIN; VIRUS VECTOR;

ANIMAL CELL; ARTICLE; BACULOVIRUS; CELL LINE; CONTROLLED STUDY; LEPIDOPTERA; MOSAIC VIRUS; NONHUMAN; PLANT VIRUS; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; SEDIMENTATION RATE; SUCROSE GRADIENT; VIRION; VIRUS ASSEMBLY; VIRUS MORPHOGENESIS; VIRUS MORPHOLOGY; VIRUS PARTICLE;

SPODOPTERA FRUGIPERDA;

EID: 0034529270     PISSN: 00221317     EISSN: None     Source Type: Journal    
DOI: 10.1099/0022-1317-81-12-3093     Document Type: Article

References (15)

1. Transgenic plants and insect cells expressing the coat proteins of arabis mosaic virus produce empty virus-like particles
2. Studies on hybrid comoviruses reveal the importance of three-dimensional structure for processing of the viral coat proteins and show that the specificity of cleavage is greater in trans than in cis
3. Expression of middle-component RNA of cowpea mosaic virus: In vitro generation of a precursor to both capsid proteins by a bottom-component RNA-encoded protease from infected cells
4. Comoviruses: Molecular biology and replication
5. Cleavage of the structural proteins during the assembly of bacteriophage T4
6. The refined crystal structure of cowpea mosaic virus at 2·8 A resolution
7. The synthesis and structure of comovirus capsids
8. Expression of cowpea mosaic virus coat protein precursor in transgenic tobacco plants
9. Identification of a discrete intermediate in the assembly/ disassembly of physalis mottle tymovirus through mutational analysis
10. Expression of tobacco ringspot virus capsid protein and satellite RNA in insect cells and three-dimensional structure of tobacco ringspot virus-like particles
11. Cell-to-cell transport of cowpea mosaic virus requires both the 58/48K proteins and the capsid proteins
12. Capsid proteins of cowpea mosaic virus transiently expressed in protoplasts form virus-like particles
13. Two proteins from cowpea mosaic virus
14. Purification, characterization, assembly and crystallization of assembled alfalfa mosaic virus coat protein expressed Escherichia coli
15. In vitro assembly of cowpea chlorotic mottle virus from coat protein expressed in Escherichia coli and in vitro transcribed viral cDNA