Production of stable transgenic sunflowers (Helianthus annuus L.) from wounded immature embryos by particle bombardment and co-cultivation with Agrobacterium tumefaciens

Molecular Breeding

Volumn 6, Issue 5, 2000, Pages 479-487

  Lucas, O ^a   Kallerhoff, J ^a   Alibert, G ^a  

^a UNIVERSITÉ DE TOULOUSE   (France)

Author keywords

Agrobacterium tumefaciens; Genetic transformation; GUS expression; Helianthus annuus L.; Immature embryos; Transgenic progeny

Indexed keywords

BACTERIAL GENE; BINARY VECTOR; GENE INSERTION; GENE SEGREGATION; GENETIC STRAIN; GENETIC TRANSFORMATION; MICROPROJECTILE BOMBARDMENT; PROGENY; SHOOT GROWTH; SOUTHERN BLOTTING; TRANSGENIC PLANT;

BACTERIA; NUCLEIC ACIDS; PLANTS (BOTANY);

AGROBACTERIUM TUMEFACIENS; CO-CULTIVATION; EMBRYONIC AXES; GENETIC TRANSFORMATION; GUS EXPRESSION; HELIANTHUS ANNUUS L; IMMATURE EMBRYOS; PARTICLE BOMBARDMENT; TRANSGENIC PROGENY; TRANSGENICS;

GENES;

AGROBACTERIUM TUMEFACIENS; HELIANTHUS ANNUUS;

EID: 0034526375     PISSN: 13803743     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1026583931327     Document Type: Article

References (14)

1. Microprojectile bombardment of plant tissues increases transformation frequency by Agrobacterium tumefaciens
2. Agrobacterium-mediated transformation of sunflower (Helianthus annuus L.) shoot apices: Transformation patterns
3. Genetic engineering of sunflower (Helianthus annuus L.)
4. Microprep protocol for extraction of DNA from tomato and other herbaceous plants
5. Assaying chimeric genes in plants: The GUS gene fusion system
6. Transformation of sunflower (Helianthus annuus L.): A reliable protocol
7. Expression of foreign genes in sunflower (Helianthus annuus L.): Evaluation of three transfer methods
8. Stable transformation of sunflower using Agrobacterium and split embryonic axis explants
9. A revised medium for rapid growth and bioassays with tobacco tissue cultures
10. Optimising the biolistic process for different biological applications
11. Meristem transformation of sunflower via Agrobacterium
12. Transformation of plants via the shoot apex
13. Construction of an intron-containing marker gene: Splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacteriwn-mediated plant transformation