The role of conserved His775/781 in membrane-binding PQQ glucose dehydrogenase of Escherichia coli and Acinetobacter calcoaceticus

Journal of Biochemistry, Molecular Biology and Biophysics

Volumn 4, Issue 6, 2000, Pages 415-422

  Okuda, J ^a   Yoshida, H ^a   Kojima, K ^a   Himi, M ^a   Sode, K ^a  

^a TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY   (Japan)

Author keywords

PQQ glucose dehydrogenase; Substrate specificity; Thermal stability

Indexed keywords

GLUCOSE DEHYDROGENASE; HISTIDINE; PYRROLOQUINOLINEQUINONE;

ACINETOBACTER CALCOACETICUS; ARTICLE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME SPECIFICITY; ESCHERICHIA COLI; MEMBRANE BINDING; NONHUMAN; THERMOSTABILITY;

EID: 0034524811     PISSN: 10258140     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. Glu742 substitution to Lys enhances the EDTA tolerance of Escherichia coli PQQ glucose dehydrogenase
2. Elucidation of the region responsible for EDTA tolerance in PQQ glucose dehydrogenases by constructing Escherichia coli and Acinetobacter calcoaceticus chimeric enzymes
3. Construction and characterization of a chimeric Escherichia coli PQQ glucose dehydrogenase (PQQGDH) with increased EDTA tolerance
4. Engineering a chimeric pyrroloquinoline quinone glucose dehydrogenase: Improvement of EDTA tolerance, thermal stability and substrate specificity
5. Thermostable chimeric PQQ glucose dehydrogenase
6. Site-directed mutagenesis study on the thermal stability of a chimeric PQQ glucose dehydrogenase and its structural interpretation
7. Topological analysis of quinoprotein glucose dehydrogenase in Escherichia coli and its ubiquinone-binding site
8. Secondary structure study of pyrroloquinoline quinone glucose dehydrogenase
9. Structure of the quinoprotein glucose dehydrogenase in Escherichia coli modeled on that of methanol dehydrogenase from Methylobacterium extorquens
10. Single amino acid substitution changes the substrate specificity of quinoprotein glucose dehydrogenase in Gluconobacter oxydans
11. Improved substrate specificity and dynamic range for glucose measurement of Escherichia coli PQQ glucose dehydrogenase by site directed mutagenesis
12. Mutant isolation of Escherichia coli quinoprotein glucose dehydrogenase and analysis of crucial residues Asp-730 and His-775 for its function
13. Cloning, mapping and sequencing of the gene encoding Escherichia coli quinoprotein glucose dehydrogenase
14. Mode of binding of pyrroloquinoline quinone to apo-glucose dehydrogenase
15. Purification and characterization of quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus L.M.D.79.41
16. Thr424 to Asn substitution alters bivalent metal specificity of pyrroloquinoline quinone glucose dehydrogenase