Time-resolved fluorescence reveals two binding sites of 1,8-ANS in intact human oxyhemoglobin

Journal of Photochemistry and Photobiology B: Biology

Volumn 58, Issue 2-3, 2000, Pages 156-162

  Parul, D A ^a   Bokut, S B ^a   Milyutin, A A ^a   Petrov, E P ^b   Nemkovich, N A ^b   Sobchuk, A N ^b   Dzhagarov, B M ^b  

^a International Sakharov Environmental University   (Belarus)

^b B I STEPANOV INSTITUTE OF PHYSICS   (Belarus)

Author keywords

1,8 Anilinonaphthalene sulfonate; Fluorescence decay time distribution; Fluorescent probe; Intact human oxyhemoglobin

Indexed keywords

8 ANILINO 1 NAPHTHALENESULFONIC ACID; BUFFER; FLUORESCENT DYE; OXYHEMOGLOBIN; POTASSIUM; WATER;

ANALYSIS; ARTICLE; BINDING SITE; CONTROLLED STUDY; EXCITATION; FEMALE; FLUORESCENCE; HUMAN; HUMAN TISSUE; KINETICS; NORMAL HUMAN; PH; POLARIZATION; PRIORITY JOURNAL; SPECTRAL SENSITIVITY; TIME;

ANILINO NAPHTHALENESULFONATES; BINDING SITES; FEMALE; FLUORESCENT DYES; HUMANS; OXYHEMOGLOBINS; PHOTOCHEMISTRY; SOLUTIONS; SPECTROMETRY, FLUORESCENCE;

EID: 0034508684     PISSN: 10111344     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1011-1344(00)00122-6     Document Type: Article

References (36)

1. Intrinsic fluorescence of hemoglobins and myoglobins
2. The interaction of a naphthalene dye with apomyoglobin and apohemoglobin. A fluorescent probe of non-polar binding sites
3. 137 Cs
4. Kinetic parameters of human hemoglobin oxygenation in the presence of the fluorescent probe 1,8-anilinonaphthalene sulfonate
5. Spectrophotometric studies. XIV. Crystallographic and optical properties of the hemoglobin of man in comparison with those of other species
6. Studies on the heterogeneity of hemoglobin. IX. The use of tris(hydroxymethyl)aminomethane-HCI buffer in the anion-exchange chromatography of hemoglobins
7. Automated spectrofluorimeter for biology and medicine
8. Effect of the external refractive index on fluorescence kinetics of perylene in human erythrocyte ghosts
9. Generalized cross-validation as a method for choosing a good ridge parameter
10. Improved recursive convolution integral for the analysis of fluorescence decay data: Local approximation of the apparatus response function by a general polynomial
11. Dipole moments of phenylnaphtylamine fluorescence probes and study of dielectric interactions in human erythrocyte ghosts
12. Electron hydration dynamics using 2-anilinonaphthalene precursor
13. Excited state electron and proton transfers
14. Study of solvent effects on the fluorescence of 1-(dimethylamino)-5-naphthalenesulfonic acid and related compounds
15. The influence of solvent-solute interaction on radiationless processes: Excited state dynamics of 1,8-anilinonaphthalene sulfonate and related molecules
16. Role of specific solvation in the fluorescence sensitivity of 1,8-ANS to water
17. Self-association of 8-anilinonaphthalene-sulfonate molecules: Spectroscopic characterization and application to the investigation of protein folding
18. Intramolecular donor-acceptor systems. 10. Multiple fluorescence from 8-(N-phenylamino)-1-naphthalenesulfonates
19. Picosecond studies of the fluorescence probe molecule 8-anilino-1-naphthalenesulfonic acid
20. Specialized functional domains in hemoglobin: Dimensions in solution of the apohemoglobin dimer labeled with fluorescein iodoacetamine
21. Inhomogeneous broadening of electronic spectra of dye molecules in solutions
22. Fluorescence decay time distribution for polar dye solutions with time-dependent fluorescent shift
23. Nanosecond decay studies of fluorescence probe bound to apomyoglobin
24. Binding of naphthalene dyes to the N and A conformers of bovine α-lactalbumin
25. Human aglycosyl-IgG exhibits increased hydrophobicity: Binding fluorescence studies with 8-anilinonaphthalene-1-sulfonic acid (ANS)
26. Localization of the 1-anilino-8-naphthalenesulfonate fluorescent probe on the surface monolayer of low density lipoprotein
27. Anion-binding sites of human serum albumin at the N-F conformational transition in isolated albumin and blood serum
28. Use of fluorescence decay times of 8-ANS-protein complexes to study the conformational transitions in proteins which unfold through the molten globute state
29. Headgroup hydration and motional order of lipids in lamellar liquid crystalline and inverted hexagonal phases of unsaturated phosphatidylethanolamine - A time-resolved fluorescence study