A simple and rapid method for lithium acetate-mediated transformation of Kluyveromyces marxianus cells

World Journal of Microbiology and Biotechnology

Volumn 16, Issue 7, 2000, Pages 653-654

  Antunes, D F ^a   De Souza C G , Jr ^a   De Morais M A , Jr ^a  

^a FEDERAL UNIVERSITY OF PERNAMBUCO   (Brazil)

Author keywords

Dithiothreitol; Kluyveromyces marxianus; Lithium acetate; Yeast transformation

Indexed keywords

CELL TRANSFORMATION; FUNGAL DNA; FUNGUS CELL; GENETIC TRANSFORMATION; HEAT SHOCK; LITHIUM ACETATE; PLASMID; TEMPERATURE;

KLUYVEROMYCES MARXIANUS;

EID: 0034494034     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1008984403732     Document Type: Article

References (10)

1. Production of bioingredients from Kluyveromyces marxianus grown on whey: An alternative
2. Expression of an α-galactosidase gene under control of the homologous inulinase promoter in Kluyveromyees marxianus
3. One-step transformation of yeast in stationary phase
4. DMSO-enhanced whole cell yeast transformation
5. High efficiency transformation of Kluyveromyces marxianus by a replicative plasmid
6. Factors influencing deactivation of yeast cells exposed to ethanol
7. Ethanol improves the transformation efficiency of intact yeast cells
8. High efficiency transformation of intact yeast cells by electric field pulses
9. Parameters affecting lithium acetate-mediated transformation of Saccharomyces cerevisiae and development of a rapid and simplified procedure
10. The use of the replicating pDblet plasmid as a cloning vector with enhanced stability in Kluyveromyces marxianus