Electroporation of intact embryonic leaflets of peanut: Gene transfer and stimulation of regeneration capacity

In Vitro Cellular and Developmental Biology - Plant

Volumn 36, Issue 5, 2000, Pages 374-378

  De Padua V L M ^a   Pestana, M C ^a   Margis Pinheiro, M ^a   De Oliveira, D E ^a   Mansur, E ^b  

^a FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

^b STATE UNIVERSITY OF RIO DE JANEIRO   (Brazil)

Author keywords

Arachis hypogaea; Intact tissue; Organogenesis; Transformation; Transient expression

Indexed keywords

ANTIBIOTIC RESISTANCE; BETA GLUCURONIDASE; BUFFER; ELECTROPORATION; EMBRYO; ENZYME ASSAY; GENE TRANSFER; IN VITRO CULTURE; PEANUT; PLANT LEAF; SHOOT REGENERATION;

EID: 0034492716     PISSN: 10545476     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11627-000-0067-6     Document Type: Article

References (28)

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4. Expression and inheritance of foreign genes in transgenic peanut plants generated by Agrobacterium-mediated transformation
5. Evaluation of peanut (Arachis hypogaea L.) leaflets from mature zygotic embryos as recipient tissue of biolistic gene transfer
6. Transgenic maize plants by tissue electroporation
7. Transient gene expression in intact and organized rice tissues
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13. Assaying chimeric genes in plants: The GUS gene fusion system
14. Gene transfer by electroporation into intact scutellum cells of wheat embryos
15. Transient expression of GUS and the 2S albumin gene from Brazil nut in peanut (Arachis hypogaea L.) seed explants using particle bombardment
16. Production of fertile transgenic maize by electroporation of suspension culture cells
17. Improved electroporation buffer enhances transient gene expression in Arachis hypogaea protoplasts
18. Peanut transformation
19. Regeneration of peanut (Arachis hypogaea L.) plantlets by in vitro culture of immature leaves
20. A revised medium for rapid growth and bioassays with tobacco tissue cultures
21. Rapid isolation of high molecular weight plant DNA
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24. Optimization of conditions for electroporation and transient expression of foreign genes in sugarcane protoplasts
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26. Expression of a Bacillus thuringiensis cryIA(c) gene in transgenic peanut plants and its efficacy against lesser cornstalk borer
27. Construction of an intron-containing marker gene: Splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformation