Partial purification of Cry 1A toxin-binding proteins from Helicoverpa armigera larval midgut

World Journal of Microbiology and Biotechnology

Volumn 16, Issue 7, 2000, Pages 635-640

  Bagchi, T ^a  

^a M S UNIVERSITY OF BARODA   (India)

Author keywords

Bacillus thuringiensis; Cry 1Ab binding proteins; Cry 1Ab toxin; Cry 1Ac binding proteins; Cry 1Ac toxin; Helicoverpa armigera; Soybean agglutinin affinity chromatography

Indexed keywords

AFFINITY LABELING; BACTERIAL PROTEIN; CHROMATOGRAPHY; CYTOTOXICITY; ENDOTOXIN; GENETIC STRAIN; GENETIC SUSCEPTIBILITY; INDIA; PROTEIN BINDING; PROTEIN PURIFICATION; SOYBEAN AGGLUTININ;

EID: 0034491718     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1008915913590     Document Type: Article

References (24)

1. Cloning and characterization of Manduca sexta and Plutella xylostella aminopeptidase N enzymes related to Bacillus thuringiensis toxin-binding proteins
2. Identification of putative brush border membrane binding molecules specific to Bacillus thuringirensis delta-endotoxin by protein blot analysis
3. Identification, isolation and cloning of a Bacillus thuringiensis Cry 1Ac toxin binding protein from the midgut of the lepidopteran insect Heliothis virescens
4. Specificity of Bacillus thuringiensis delta-endotoxin is correlated with the presence of high affinity binding sites in the brush border membrane target insect midguts
5. Ligand specificity and affinity of BT-R1, the Bacillus thuringiensis Cry 1A toxin receptor from Manduca sexta, expressed in mammalian and insect cell cultures
6. Effects of midgut-protein preparative and ligand binding procedures on the toxin binding characteristics of BT-R1, a common high-affinity recepor in Manduca sexta for Cry 1A Bacillus thuringiensis toxins
7. The receptor for Bacillus thuringiensis Cry 1Ac delta-endotoxin in the brush border membrane of the lepidopteran Manduca sexta is aminopeptidase N
8. Molecular cloning of an insect aminopeptidase N that serves as a receptor for Bacillus thuringiensis Cry 1Ac toxin
9. Aminopeptidase N purified from Gypsy moth brush border membrane vesicles is a specific receptor for Bacillus thuringiensis Cry 1Ac toxin
10. The Heliothis virescens 170 kDa aminopeptidase functions as 'receptor A' by mediating specific Bacillus thuringiensis Cry 1A delta-endotoxin binding and pore formation
11. Binding of Bacillus thuringiensis Cry 1Ac toxin to aminopeptidase in susceptible and resistant diamond back moth (Plutella xylostella)
12. Binding of Bacillus thuringiensis proteins to a laboratory selected line of Heliothis virescens
13. A convenient procedure for the preparation of highly purified parasporal crystals of Bacillus thuringiensis
14. Insect resistance to the biological insecticide Bacillus thuringiensis
15. Partial purification and characterization of Bacillus thuringiensis Cry 1A toxin receptor A from Heliothis virescens and cloning of the corresponding cDNA
16. Proteinase-mediated insect resistance to Bacillus thuringiensis toxin
17. The insecticidal crystal protein Cry 1Ac from Bacillus thuringiensis is highly toxic for Heliothis armigera
18. Progress and prospects for field use of Bt genes in crops
19. Resistance ora diamond back moth (Lepidoptera: Plutelladae) to Bacillus thuringiensis subspecies in the field
20. A specific binding protein from Manduca sexta for the insecticidal toxin of Bacillus thuringiensis subsp, berliner
21. Cloning and expression of a receptor for an insecticidal toxin of Bacillus thuringiensis
22. Receptors on the brush border membrane of the insect midgut as determinants of the specificity of Bacillus thuringiensis delta-endotoxin
23. Mechanism of insect resistance to the microbial insecticide Bacillus thuringiensis
24. Preparation and partial characterization of amino acid transporting brush border membrane vesicles from the larval midgut of the cabbage butterfly (Pieris brassicae)