Amplification of the 23S rRNA gene and its application in differentiation and detection of phytoplasmas

Canadian Journal of Plant Pathology

Volumn 22, Issue 4, 2000, Pages 380-386

  Guo, Y H ^a   Cheng, Z M ^a   Walla, J A ^a  

^a NORTH DAKOTA STATE UNIVERSITY   (United States)

Author keywords

23S rRNA gene and sequence; PCR RFLP; Phytoplasmas; X disease

Indexed keywords

GENETIC STRAIN; OLIGONUCLEOTIDE; PHYTOPLASMA; PRIMER RNA; RESTRICTION ENDONUCLEASE; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; RIBOSOME RNA; RNA AMPLIFICATION; SEQUENCE HOMOLOGY;

EID: 0034479530     PISSN: 07060661     EISSN: None     Source Type: Journal    
DOI: 10.1080/07060660009500456     Document Type: Article

References (20)

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2. Chromosomal organization and nucleotide sequence of the gene coding for elongation factors G and Tu of the apple proliferation phytoplasma
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4. Cluster-specific polymerase chain reaction amplification of 16S rDNA sequences for detection and identification of mycoplasmalike organisms
5. Amplification of 16S rRNA genes from culturable and nonculturable mollicutes
6. Incidence of yellows in carrot and lettuce and characterization of mycoplasmalike organism isolates in Oklahoma
7. Use of polymerase chain reaction to produce oligonucleotide probes for mycoplasmalike organisms
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9. Genomic diversity and differentiation among phytoplasma strains in 16S rRNA group I (aster yellows and related phytoplasmas) and III (X-disease and related phytoplasmas)
10. X-disease confirmation and distribution in chokecherry in North Dakota
11. Phylogenetic relationships between the western aster yellows mycoplasmalike organism and other prokaryotes established by 16S rRNA gene sequence
12. Universal amplification and analysis of pathogen 16S rDNA for classification and identification of mycoplasmalike organisms
13. Use of mycoplasmalike organism (MLO) group-specific oligonucleotide primers for nested-PCR assays to detect mixed-MLO infection in a single host plant
14. Revised classification scheme of phytoplasmas based on RFLP analyses of 16S rRNA and ribosomal protein gene sequences
15. 16S rRNA sequence indicates that plant-pathogenic mycoplasmalike organisms are evolutionarily distinct from animal mycoplasmas
16. Evolutionary relationships of a plant-pathogenic mycoplasmalike organism and Acholeplasma laidlawii deduced from two ribosomal protein gene sequences
17. Leafhopper vectors of the peach X-disease pathogen and its seasonal transmission from chokecherry
18. Comparative analysis of 16S and 23S rRNA sequences of Listeria species
19. Phylogenetic classification of phytopathogenic mollicutes by sequence analysis of 16S ribosomal DNA
20. Phytoplasma-specific PCR primers based on sequences of the 16S-23S rRNA spacer region