Whole-mount confocal immunofluorescence of mammalian CNS

Brain Research Protocols

Volumn 6, Issue 3, 2001, Pages 129-133

  Luque, J M ^a   Morante Oria, J ^a   Riederer, B M ^b   Fairen A ^a  

^a MIGUEL HERNÁNDEZ UNIVERSITY   (Spain)

^b Institut de Biologie Cellulaire et de Morphologie   (Switzerland)

Author keywords

Calbindin calretinin; Cortex; Developmental neurobiology; Neurofilament; Rat

Indexed keywords

ANTIBODY; CALCIUM BINDING PROTEIN; CYTOSKELETON PROTEIN;

ANIMAL TISSUE; ARTICLE; CENTRAL NERVOUS SYSTEM; CONTROLLED STUDY; EMBRYO; IMMUNOFLUORESCENCE TEST; LASER MICROSCOPY; MAMMAL; NERVE CELL; NERVE FIBER; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; RAT; THREE DIMENSIONAL IMAGING;

ANIMALS; ANTIBODIES; BRAIN CHEMISTRY; CALCIUM-BINDING PROTEIN, VITAMIN D-DEPENDENT; CEREBRAL CORTEX; FEMALE; FETUS; FLUORESCENT ANTIBODY TECHNIQUE; MAMMALS; MICROSCOPY, CONFOCAL; NEUROFILAMENT PROTEINS; PREGNANCY; RATS; RATS, WISTAR;

EID: 0034473047     PISSN: 1385299X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1385-299X(00)00045-3     Document Type: Article

References (16)

1. The development of the septal region in the rat
2. Multicolor laser scanning confocal immunofluorescence microscopy: Practical application and limitations
3. A whole mount immunocytochemical analysis of the expression of the intermediate filament protein vimentin in Xenopus
4. A study in developing visual systems with a new method of staining neurones and their processes in fixed tissue
5. Three-dimensional analysis of the developing pituitary in the mouse
6. Fluorescent carbocyanine dyes allow living neurons of identified origin to be studied in long-term cultures
7. Whole-mount staining of xenopus and other vertebrates
8. Prenatal development of nucleus basalis complex and related fiber systems in man: A histochemical study
9. A note on the preparation of whole mount samples suitable for observation with the confocal laser scanning microscope
10. Procedures for whole-mount immunohistochemistry and in situ hybridization of immature mammalian CNS
11. Different origins and developmental histories of transient neurons in the marginal zone of the fetal and neonatal rat cortex
12. Mechanisms underlying the early establishment of thalamocortical connections in the rat
13. Solubility of cytoskeletal proteins in immunohistochemistry and the influence of fixation
14. Cellular mosaics in the rat marginal zone define an early neocortical territorialization
15. Preparation, staining and examination of nervous tissue in the confocal microscope
16. Two-laser dual-immunofluorescence confocal laser scanning microscopy using Cy2- and Cy5-conjugated secondary antibodies: Unequivocal detection of co-localization of neuronal markers