Modest increases in the titers of helper virus-free herpes simplex virus 1 (HSV-1) vectors by packaging in a cell line with inducible expression of HSV-1 VP16 or by treatment with N,N′-hexamethylene-bis-acetamide

Acta Virologica

Volumn 44, Issue 6, 2000, Pages 365-369

  Sun, M ^a   Lee, J ^a   Yu, L ^a   Geller, A I ^a  

^a BOSTON CHILDREN S HOSPITAL   (United States)

Author keywords

Helper virus free packaging; HMBA; HSV 1 vector; VP16

Indexed keywords

ETOPOSIDE; HEXAMETHYLENEBISACETAMIDE; TETRACYCLINE; VIRUS VECTOR;

ANIMAL CELL; ARTICLE; CELL LINE; CONTROLLED STUDY; GENE EXPRESSION; GENE INDUCTION; GENE THERAPY; HELPER VIRUS; HERPES SIMPLEX VIRUS 1; NONHUMAN; PROMOTER REGION; VERO CELL; VIRUS EXPRESSION; VIRUS TITRATION;

ACETAMIDES; ANIMALS; CERCOPITHECUS AETHIOPS; GENETIC VECTORS; HELPER VIRUSES; HERPES SIMPLEX VIRUS PROTEIN VMW65; HERPESVIRUS 1, HUMAN; TRANSDUCTION, GENETIC; VERO CELLS; VIRUS ASSEMBLY;

ANIMALIA; HERPES; HUMAN HERPESVIRUS 1;

EID: 0034464594     PISSN: 0001723X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. Mutational analysis of the herpes simplex virus type 1 trans-inducing factor Vmw65
2. Efficacy of tetracycline-controlled gene expression is influenced by cell type
3. A cosmid-based system for constructing mutants of herpes simplex virus type 1
4. PREPs: Herpes simplex virus type 1-specific particles produced by infected cells when viral DNA replication is blocked
5. Helper virus-free transfer of herpes simplex virus type 1 plasmid vectors into neural cells
6. Genetic analysis of the role of protein kinase C signaling pathways in behaviors by direct gene transfer with HSV-1 vectors
7. A defective HSV-1 vector expresses Escherichia coli beta-galactosidase in cultured peripheral neurons
8. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters
9. Size and location of the transforming region in human adenovirus type 5 DNA
10. Allele replacement: An application that permits rapid manipulation of herpes simplex virus type 1 genomes
11. Cytotoxicity of a replication-defective mutant of herpes simplex virus type 1
12. A simple assay for DNA transfection by incubation of the cells in culture dishes with substrates for beta-galactosidase
13. Hexamethylene bisacetamide stimulates herpes simplex virus immediate early gene expression in the absence of trans-induction by Vmw65
14. The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1
15. Herpes simplex viruses and their replication
16. Herpes simplex virus type 1 DNA amplified as bacterial artificial chromosome in Escherichia coli: Rescue of replication-competent virus progeny and packaging of amplicon vectors
17. Evidence that the herpes simplex virus immediate early protein ICP27 acts post-transcriptionally during infection to regulate gene expression
18. Long-term expression in sensory neurons in tissue culture from herpes simplex virus type 1 (HSV-1) promoters in an HSV-1-derived vector
19. An enhanced packaging system for helper-dependent herpes simplex virus vectors
20. Improved titers for helper virus-free herpes simplex virus type 1 plasmid vectors by optimization of the packaging protocol and addition of noninfectious herpes simplex virus-related particles (previral DNA replication enveloped particles) to the packaging procedure
21. Inflammatory effects of gene transfer into the CNS with defective HSV-1 vectors