An intronic splicing enhancer binds U1 snRNPs to enhance splicing and select 5′ splice sites

Molecular and Cellular Biology

Volumn 20, Issue 24, 2000, Pages 9225-9235

  McCullough, A J ^a   Berget, S M ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA PRECURSOR; RNA BINDING PROTEIN; SMALL NUCLEAR RIBONUCLEOPROTEIN;

ARTICLE; CONTROLLED STUDY; HELA CELL; HUMAN; HUMAN CELL; INTRON; MOLECULAR INTERACTION; MOLECULAR RECOGNITION; PRIORITY JOURNAL; PROTEIN DNA INTERACTION; PROTEIN RNA BINDING; RNA SPLICING;

BASE PAIRING; BASE SEQUENCE; CROSS-LINKING REAGENTS; ELECTROPHORESIS; ENHANCER ELEMENTS (GENETICS); FICUSIN; GLOBINS; HELA CELLS; HUMANS; INTRONS; MOLECULAR SEQUENCE DATA; MUTATION; NUCLEAR PROTEINS; NUCLEIC ACID CONFORMATION; PHOSPHOPROTEINS; RIBONUCLEOPROTEIN, U1 SMALL NUCLEAR; RNA; RNA SPLICE SITES; RNA SPLICING; RNA, SMALL NUCLEAR; RNA-BINDING PROTEINS; TRANSFECTION;

VERTEBRATA;

EID: 0034461083     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.20.24.9225-9235.2000     Document Type: Article

References (43)

1. U1, U2, and U4/U6 small nuclear ribonucleoproteins are required for in vitro splicing but not polyadenylation
2. Activation of c-src neuron-specific splicing by an unusual RNA element in vivo and in vitro
3. U2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicing
4. Modulation of exon skipping by high-affinity hnRNP A1-binding sites and by intron elements that repress splice site utilization
5. Regulation of alternative splicing in vivo by overexpression of antagonistic splicing factors
6. An intron splicing enhancer containing a G-rich repeat facilitates inclusion of a vertebrate micro-exon
7. An intron element modulating 5′ splice site selection in the hnRNP A1 pre-mRNA interacts with hnRNP A1
8. U1 small nuclear RNAs with altered specificity can be stably expressed in mammalian cells and promote permanent changes in pre-mRNA splicing
9. Complementation by SR proteins of pre-mRNA splicing reactions depleted of U1 snRNP
10. Cis-acting elements distinct from the 5′ splice site promote U1- independent pre-mRNA splicing
11. A short sequence within two purine-rich enhancers determines 5′ splice site specificity
12. Pathways for selection of 5′ splice sites by U1 snRNPs and SF2/ASF
13. Improved free-energy parameters for predictions of RNA duplex stability
14. Specific commitment of different pre-mRNAs to splicing by single SR proteins
15. Sequences homologous to 5′ splice sites are required for the inhibitory activity of papillomavirus late 3′ untranslated regions
16. Characterization of RNA
17. U1 snRNP inhibits pre-mRNA polyadenylation through a direct interaction between U1 70K and poly(A) polymerase
18. Intron definition is required for excision of the minute virus of mice small intron and definition of the upstream exon
19. Regulation of sex-specific selection of fruitless 5′ splice sites by transformer and transformer-2
20. Association of U2 snRNP with the spliceosomal complex E
21. U1 snRNA promotes the selection of nearby 5′ splice sites by U6 snRNA in mammalian cells
22. U1 snRNP-ASF/SF2 interaction and 5′ splice site recognition: Characterization of required elements
23. Regulation of alternative polyadenylation by U1 snRNPs and SRp20
24. Regulation of alternative pre-mRNA splicing by hnRNP A1 and splicing factor SF2
25. Characterization of an intron splice enhancer that regulates alternative splicing of human GH pre-mRNA
26. G triplets located throughout a class of small vertebrate introns enforce intron borders and regulate splice site selection
27. U1 small nuclear ribonucleoprotein and splicing inhibition by the Rous sarcoma virus negative regulator of splicing element
28. An ATP-independent complex commits pre-mRNA to the mammalian spliceosome assembly pathway
29. The U1 small nuclear RNA-protein complex selectively binds a 5′ splice site in vitro
30. Conserved quartets near 5′ intron junctions in primate nuclear pre-mRNA
31. Association of U6 snRNA with the 5′-splice site region of pre-mRNA in the spliceosome
32. Split genes and RNA splicing
33. The mechanism of somatic inhibition of Drosophila P-element pre-mRNA splicing: Multiprotein complexes at an exon pseudo-5′ splice site control U1 snRNP binding
34. An intronic (A/U)GGG repeat enhances the splicing of an alternative intron of the chicken beta-tropomyosin pre-mRNA
35. Predicting internal exons by oligonucleotide composition and discriminant analysis of spliceable open reading frames
36. SR proteins promote the first specific recognition of pre-mRNA and are present together with the U1 small nuclear ribonucleoprotein particle in a general splicing enhancer complex
37. SR proteins can compensate for the loss of U1 snRNP functions in vitro
38. Overexpression of the SR proteins ASF/SF2 and SC35 influences alternative splicing in vivo in diverse ways
39. Interactions of small nuclear RNA's with precursor messenger RNA during in vitro splicing
40. The role of exon sequences in splice site selection
41. Site-specific cross-linking of mammalian U5 snRNP to the 5′ splice site before the first step of pre-mRNA splicing
42. A compensatory base change in U1 snRNA suppresses a 5′ splice site mutation
43. The human splicing factor ASF/SF2 can specifically recognize pre-mRNA 5′ splice sites