Human anti-mouse antibodies: Pitfalls in tumor marker measurement and strategies for enhanced assay robustness; including results with Elecsys® CEA

Anticancer Research

Volumn 20, Issue 6 D, 2000, Pages 5249-5252

  Nussbaum, S ^a   Roth, H J ^a  

^a ROCHE DIAGNOSTICS GMBH   (Germany)

Author keywords

Blocking reagents; Chimerisation; HAMA interference; Interference elimination

Indexed keywords

ANTIBODY; CA 125 ANTIGEN; CA 19-9 ANTIGEN; CA 72-4 ANTIGEN; CARCINOEMBRYONIC ANTIGEN; CHIMERIC ANTIBODY; HUMAN ANTI MOUSE ANTIBODY; MONOCLONAL ANTIBODY; THYROTROPIN; TUMOR MARKER; UNCLASSIFIED DRUG;

CHIMERA; CONFERENCE PAPER; DIAGNOSTIC ERROR; DIAGNOSTIC TEST; DIAGNOSTIC VALUE; HUMAN; MEASUREMENT; PRIORITY JOURNAL;

ANIMALS; ANTIBODIES; ANTIGENS, TUMOR-ASSOCIATED, CARBOHYDRATE; CA-125 ANTIGEN; CA-19-9 ANTIGEN; CARCINOEMBRYONIC ANTIGEN; DIAGNOSTIC TECHNIQUES AND PROCEDURES; HUMANS; MICE; QUALITY CONTROL; REAGENT KITS, DIAGNOSTIC; RECOMBINANT FUSION PROTEINS; TUMOR MARKERS, BIOLOGICAL;

EID: 0034450615     PISSN: 02507005     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Conference Paper

References (7)

1. Human anti-animal antibody interferences in immunological assays
2. Value od HAMA-determination in clinical practice - An overview
3. Reaction profiles of seven enzyme immunoassay kits for carcinoembryonic antigen (CEA) analysed with purified preparations of CEA and related normal antigens
4. Reducing interference from heterophilic antibodies in a two-site immunoassay for carcinoembryonic antigen (CEA) by using a human / mouse chimeric antibody to CEA as the tracer
5. Eliminating interference from heterophilic antibodies in a two-site immunoassay for creatine kinase MB by using (F(ab′)2 conjugate and polyclonal mouse IgG
6. Different efficacy of various blocking reagents to eliminate interferences by human antimouse antibodies with a two-site immunoassay
7. Significance of the tumor markers CA 125II, CA 72-4, CASA and Cyfra 21-1 in ovarian carcinoma