Measurement of diffusivity of cell-wall degrading enzymes in food-agarose gels

Deutsche Lebensmittel-Rundschau

Volumn 96, Issue 4, 2000, Pages 139-142

  Varzakas, Theodoros H ^a,b  

^a UNIVERSITY OF READING   (United Kingdom)

^b NONE   (Greece)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0034419598     PISSN: 00120413     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (10)

1. Schmidt, O., and U. A. Kebernik: Simple assay with dyed substrates to quantify cellulase and hemicellulase activity of fungi. Biotechnol. Tech., 2, 153-158 (1988)
2. Kauri, T., and D. J. Kushner: Detection of cellulolytic activity of bacteria and fungi growing on agar surfaces. Biotechnol. Tech., 2, 149-152 (1988).
3. Mathew, R., and K. K. Rao: Activity staining of endoglucanases in polyacrylamide gels. Anal. Biochem. 206, 50-52 (1992).
4. Beguin, P.: Detection of cellulase activity in polyacrylamide gels using Congo-Red-Stained agar replicas. Anal. Biochem. 131, 333-336 (1983).
5. Teather, R. M., and P. J. Wood: Use of Conto-Red-Polysaccharide Interactions in Enumeration and characterisation of cellulolytic bacteria from the bovine rumen. Appl. Environ. Microbiol. 43, 777-780 (1982).
6. Biely, P., M. Vrsanska, and S. Kucar: Identification and mode of action of endo-(1 →4)-β-xylanases. In Xylans and xylanases, ed. by J. Visser, Elsevier Science Publishers, pp. 81-95 (1992).
7. Cotty, P. J., T. E. Cleveland, R. L. Brown, and J. E. Mellon: Variation in polygalacturonase production among Aspergillus flavus isolates. Appl. Environ. Microbiol. 56, 3885-3887 (1990).
8. Cussler, E. L.: Diffusion: Mass Transfer in Fluid Systems, Cambridge University Press, Cambridge, pp. 146-177 (1984).
9. Bradford. M. M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Anal. Biochem. 72, 248-254 (1976).
10. Varzakas, T., D. L. Pyle, and K. Niranjan: A preliminary investigation on the infusion of endoglucanase and endoxylanase in soybean. In: 7th International Conference on Engineering and Food, ed. by D. Jowitt. 13-15 April, Brighton, 1997.