Purification and Characterization of Acidic Chitinases from Gizzards of Broiler (Gallus gallus L.)

Journal of Biochemistry and Molecular Biology

Volumn 33, Issue 4, 2000, Pages 326-331

  Han, Beom Ku ^a   Moon, Jong Kook ^a   Ryu, Yeon Woo ^a   Park, Yun Hee ^a   Jo, Do Hyun ^a  

^a NONE

Author keywords

Broiler; Chitinase; Gizzards; NH2 terminal; Purification

Indexed keywords

EID: 0034414956     PISSN: 12258687     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (18)

1. Collinge, D. B., Kragh, K. M., Mikkelsen, J. A., Nielsen, K. K., Rasmussen, U. and Vad, K. (1993) Plant chitinases. Plant J. 3, 31-40.
2. Flach, J., Pilet, P. E. and Jolles, P. (1992) Whats new in chitinase research? Experientia 48, 701-716.
3. Han, B. K., Lee, W. J. and Jo, D. H. (1997a) Chitinolytic enzymes from the gizzard and the chyme of the broiler (Gallus gallus L.). Biotechnol. Lett. 19, 981-984.
4. Han, B. K., Lee, W. J., You, T., Park. I. H. and Jo, D. H. (1997b) Survey on the chitinolytic activity from some plants for industrial utilization. Agric. Chem. Biotechnol. 39, 466-471.
5. Jeuniaux, C. and Cornelius, C. (1978) Distribution and activity of chitinolytic enzymes in the digestive tract of birds and mammals; In Proceedings of the first international conference on chitin/chitosan. Muzzarelli, R. A. A. and Pariser, E. R. (eds.), pp. 542-549, MIT Sea Grant Report MITSG, USA.
6. Koga, D., Jilka, J. and Kramer, K. J. (1983) Insect endochitinases: glycoproteins from moulting fluid. Integument and pupal haemolymph of Manduca sexta L. Insect Biochem. 13, 295-305.
7. Koga, D., Sasaki, Y., Uchiumi, Y., Hirai, N., Arakane, Y. and Nagamatsu, Y. (1997) Purification and characterization of Bombyx mori chitinases. Insect Biochem. Molec. Biol. 27, 757-767.
8. Kono, M., Matsui, T. and Shimizu, C. (1987) Chitin-decomposing bacteria in digestive tracts of cultured red sea bream and Japanese eel. Nippon Suisan Gakkaishi 53, 131-136.
9. Kono, M., Matsui, T., Shimizu, C. and Koga, D. (1990) Purification and some properties of chitinase from the stomach of Japanese eel. Agric. Biol. Chem. 54, 973-978.
10. Kramer, K. J. and Muthukrishnan, S. (1992) Insect chitinases: molecular biology and potential use as biopestisides. Insect Biochem. Molec. Biol. 27, 887-900.
11. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head protein of bacteriophage T4. Nature 227, 680-685.
12. Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275.
13. Lundblad, G., Elander, M., Lind, J. and Slettengren, K. (1979) Bovine serum chitinase. Eur. J. Biochem. 100, 455-460.
14. Martin, M. N. (1991) The latex of Hevea brasiliensis contains high levels of both chitinases and chitinase/lysozymes. Plant Physiol. 95, 469-476.
15. Molano, J., Duran, A. and Cabib, E. (1977) A rapid and sensitive assay for chitinase using tritiated chitin. Anal. Biochem. 83, 648-655.
16. Renkema, G. H., Boot, R. G., Muijsers, A. O., Donker-Koopman, W. E. and Aerts, J. M. F. G. (1995) Purification and characterization of human chitotriosidase, a novel member of the chitinase family of proteins. J. Biol. Chem. 275, 2198-2202.
17. Trudel, J. and Asselin, A. (1989) Detection of chitinase activity after polyacrylamide gel electrophoresis. Anal. Biochem. 178, 362-366.
18. Wray, W., Boulikas, T., Wray, V. P. and Hancock, R. (1981) Silver staining of proteins in polyacrylamide gels. Anal. Biochem. 118, 197-203.