The dimethoxytrityl resin product anchored sequential synthesis method (DMT PASS): A conceptually novel approach to oligonucleotide synthesis

Organic Process Research and Development

Volumn 4, Issue 3, 2000, Pages 214-224

  Mihaichuk, Jeffery C ^a   Hurley, T Brian ^a   Vagle, Kurt E ^a   Smith, Randall S ^a   Yegge, John A ^a   Pratt, Gretchen M ^a   Tompkins, Christopher J ^a   Sebesta, David P ^a   Pieken, Wolfgang A ^a  

^a Proligo LLC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0034395476     PISSN: 10836160     EISSN: None     Source Type: Journal    
DOI: 10.1021/op990095t     Document Type: Article

References (36)

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3. The following abbreviations are used throughout this account: ACN, acetonitrile; AcCl, acetyl chloride; Abz, 4-acetoxybenzyl; TBS, tertbutyldimethylsilyl; TBDPS, tert-butyldiphenylsilyl; DCA, dichloroacetic acid; DCM, dichloromethane; DCI, 4,5-dicyanoimidazole; DMT PASS, dimethoxytrityl resin product anchored sequential synthesis; DMF, dimethylformamide; DIPA, diisopropylamine; DIPEA, diisopropylethylamine; EtOAc, ethyl acetate; HPLC, high-pressure liquid chromatography; LC/ MS, liquid chromatography/mass spectrometric; MMT, monomethoxytrityl; NPE, 4-nitrophenethyl; TBAP, tetrabutylammonium periodate; v/v volume/volume.
4. (a) Pieken, W. P.; Gold, L. "Method for solution phase synthesis of oligonucleotides and peptides." US Patent 5,874,532, Issued February 3, 1999.
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6. Bz).
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17. Schirmeister, H.; Pfleiderer, W. Helv. Chim. Acta 1994, 77, 11. For a review on the use of the 4-nitrophenethyl protecting group in oligonucleotide chemistry, see: Pfleiderer, W.; Himmelsbach, F.; Charubala, R.; Schirmeister, H.; Beiter, A.; Schulz, B.; Trichtinger, T. Nucleosides Nucleotides 1985, 4, 81.
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