Development of molecular biological methods to analyze bacterial species diversity in freshwater and soil ecosystems

Journal of Microbiology

Volumn 38, Issue 1, 2000, Pages 11-17

  Lee, Dong Hun ^a   Noh, Sung Ae ^a   Kim, Chi Kyung ^a  

^a Chungbuk National University   (South Korea)

Author keywords

16s rRNA; Diversity analysis; PCR; Phenol degrading bacteria; SSCP

Indexed keywords

EID: 0034382621     PISSN: 12258873     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (11)

1. Amann, R.I., W. Ludwig, and K.H. Schleifer. 1995. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 59, 143-169.
2. Atlas, R.M. 1984. Diversity of microbial communities. Adv. Microb. Ecol. 7, 1-47.
3. DeLong, E.F., D.G. Franks, and A.L. Alldredge. 1993. Phylogenetic diversity of aggregate-attached vs. free-living marine bacterial assemblages. Limnol. Oceanogr. 38, 924-934.
4. Fuhrman, J.A., K. McCallum, and A.A. Davis. 1992. Novel major archaebacterial group from marine plankton. Nature 356, 148-149.
5. Gutell, R.R., N. Larsen, and C.R. Woese. 1994. Lessons from an evolving rRNA: 16S and 23S rRNA structures from a comparative perspective. Microbiol. Rev. 58, 10-26.
6. Hayashi, K. 1991. PCR-SSCP: A simple and sensitive method for detection of mutations in the genomic DNA. PCR Methods Appl. 1, 34-38.
7. Lee, D.-H., Y.-G. Zo, and S.-J. Kim. 1996. Non-radioactive method to study genetic profiles of natural bacterial communities by PCR-single-strand-conformation polymorphism. Appl. Environ. Microbiol. 62, 3112-3120.
8. Lee, S.-T., S.-K. Park, K.-H. Lee, S.A. Holmes, and R.A. Spritz. 1995. A non-radioactive method for simultaneous detection of single-strand conformation polymorphisms (SSCPs) and heteroduplexes. Mol. Cells 5, 668-672.
9. Liesack, W., and E. Stackebrandt. 1992. Occurrence of novel groups of the domain Bacteria as revealed by analysis of genetic material isolated from an Australian terrestrial environment. J. Bacterial. 174, 5072-5078.
10. Liesack, W., P.H. Janssen, F.A. Rainey, N.L. Ward-Rainey, and E. Stackebrandt. 1997. Microbial diversity in soil: the need for a combined approach using molecular and cultivation techniques, p. 375-439. In J.D. van Elsas, J.T. Trevors, and E.M.H. Wellington (eds.), Modern soil microbiology, Marcel Dekker, New York, N.Y.
11. Liu, W.-T., T.L. Marsh, H. Cheng, and L.J. Forny. 1997. Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA. Appl. Environ. Microbiol. 63, 4516-4522.