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The preparation and TEM observation of aggregates: the copolymer was first disolved in DMF. Then, water was added dropwise to the solution with stirring to induce the micellization of the PS blocks. After the water content reached ca. 95 wt%, the solutions were placed in dialysis bags to get rid of DMF and to obtain the aqueous solutions of copolymer aggregates. One drop of diluted solution was put onto a carbon-coated copper grid, and was negatively stained with uranyl acetate. The observation was conducted with a JEOL-100-CXII TEM operated at 100 kV
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note
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6 with the ICC of 2.0 wt% in DMF and diluted by a factor of 10.
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