Electrochemical detection of base pair mutation

Chemistry Letters

Volumn , Issue 9, 2000, Pages 1038-1039

  Yamashita, Kenichi ^a   Takagi, Makoto ^a   Kondo, Hiroki ^b   Takenaka, Shigeori ^a  

^a KYUSHU UNIVERSITY   (Japan)

^b KYUSHU INSTITUTE OF TECHNOLOGY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0034339947     PISSN: 03667022     EISSN: None     Source Type: Journal    
DOI: 10.1246/cl.2000.1038     Document Type: Article

References (10)

1. G. H. Keller and M. M. Manak, in "DNA probes", Stockton Press, New York (1989).
2. D. J. Caruana and A. Heller, J. Am. Chem. Soc., 121, 769 (1999).
3. S. O. Kelley, E. M. Boon, J. K. Barton, N. M. Jackson, and M. G. Hill, Nucl. Acids Res., 27, 4830 (1999).
4. S. Takenaka, Y. Uto, H. Saita, M. Yokoyama, H. Kondo, and W. D. Wilson, Chem. Commun., 1998, 1111.
5. S. Takenaka, K. Yamashita, M. Takagi, S. Uto, and H. Kondo, Anal. Chem., 72, 1334 (2000).
6. 2), kept for 2 h, and washed with Milli-Q water.
7. C. Yanisch-Perron, J. Vieira, and J. Messing, Gene, 33, 110 (1985).
8. The experiment of the crystal microbalance shows that about seven molecules of 1 are bound to one DNA duplex with 2 + 9 and 2 + 10, and these numbers were similar for the DNA duplex carrying a single mismatch in the middle of the sequence. Unexpectedly, however, the Δi values decreased in the former systems. The reason for this anomaly is not clear now, but since the terminal positions are located in the interface between the bulk solution and the DNA layer or the DNA layer and the electrode, a mismatch at these sites could affect the electron transfer reaction to the ferrocene of 1 from the electrode considerably. Although the electron flows through the array of base pairs of DNA duplex in Barton's system, the electron transfer reaction seems to occur through the pseudo-polyferrocene array covering the DNA duplex in this system.
9. W. D. Wilson, in "Nucleic Acids in Chemistry and Biology," ed. by G. M. Blackburn and M. J. Gait, Oxford University Press, New York (1996), p. 329.
10. J.-S. Sun, R. Lavery, J. Chomilier, K. Zakrzewska, T. Montenay-Garestier, and C. Helene, J. Biomol. Struct. Dyn., 9, 425 (1991).