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1
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0000115572
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T. Hirata, H. Hamada, T. Aoki, and T. Suga, Phytochemistry, 21, 2209 (1982).
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(1982)
Phytochemistry
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Hirata, T.1
Hamada, H.2
Aoki, T.3
Suga, T.4
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2
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0001841153
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T. Suga, H. Hamada, and T. Hirata, Chem. Lett., 1987, 471.
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Chem. Lett.
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Suga, T.1
Hamada, H.2
Hirata, T.3
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3
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0000748545
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T. Suga, H. Hirata, H. Hamada, and S. Murakami, Phytochemistry, 27, 1041 (1988).
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(1988)
Phytochemistry
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, pp. 1041
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Suga, T.1
Hirata, H.2
Hamada, H.3
Murakami, S.4
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4
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0001619624
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T. Hirata, Y. X. Tang, K. Okano, and T. Suga, Phytochemistry, 28, 3331 (1989).
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(1989)
Phytochemistry
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Hirata, T.1
Tang, Y.X.2
Okano, K.3
Suga, T.4
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5
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0027515354
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T. Hirata, S. Izumi, K. Shimoda, and M. Hayashi, J. Chem. Soc., Chem. Commun., 1993, 1426.
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J. Chem. Soc., Chem. Commun.
, vol.1993
, pp. 1426
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Hirata, T.1
Izumi, S.2
Shimoda, K.3
Hayashi, M.4
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6
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0002550708
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K. Shimoda, D. I. Ito, S. Izumi, and T. Hirata, J. Chem. Soc., Perkin Trans. 1, 1996, 355.
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J. Chem. Soc., Perkin Trans. 1
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Shimoda, K.1
Ito, D.I.2
Izumi, S.3
Hirata, T.4
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7
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0001967772
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note
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4 (40 to 60% satn.). The crude enzyme soin obtained was desalted and then applied to a DEAE-Toyopearl column with a 0-0.5 M linear gradient of NaCl in 50 mM Tris-HCl buffer (pH 8.0) containing 1.0 mM 2-mercaptoethanol and 1.0 mM dithiothreitol (buffer A) to give three enone reductase fractions, which contained the 44, 74 and 90 kDa proteins, respectively. Each of the fractions was further purified on a hydroxylapatite column with buffer A and a Red-Toyopearl column with buffer A containing a 0-1.0 M linear gradient of NaCl.
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9
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0001825021
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note
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The mixture (2 mL) of enone (100 μmol), NADPH (200 μmol), Triton X-100 (0.1%), and enzyme preparation (5 μg) in 50 mM Tris-HCl buffer (pH 8.0) containing 1.0 mM 2-mercaptoethanol and 1.0 mM dithiothreitol was incubated at 35 °C for 8 h. After incubation, the reaction mixture was subjected to GLC and GC-MS analyses, and then the products were purified by column chromatography on silica gel. The absolute configurations and enantiomeric purities of the products were determined by circular dichroism (CD) spectra and the peak area of the corresponding enantiomers in the GLC analyses on CP cyclodextrin β 236M-19 column.
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10
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0001941870
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note
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2-Me), 6.69 (1H, t, J = 4.3 Hz, >C=CH-).
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11
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0013287123
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F. Huet, M. Pellet, and J. M. Conia, Tetrahedron Lett., 1977, 3505.
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Tetrahedron Lett.
, vol.1977
, pp. 3505
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Huet, F.1
Pellet, M.2
Conia, J.M.3
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12
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0000227779
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K. Matsumoto, Y. Kawabata, J. Takahashi, Y. Fujita, and M. Hatanaka, Chem. Lett., 1998, 283.
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Chem. Lett.
, vol.1998
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Matsumoto, K.1
Kawabata, Y.2
Takahashi, J.3
Fujita, Y.4
Hatanaka, M.5
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14
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0001745759
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note
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288 +2480}.
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16
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33845557646
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A. I. Meyers, D. R. Williams, G. W. Erickson, S. White, and M. Druelinger, J. Am. Chem. Soc., 193, 3081 (1981).
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(1981)
J. Am. Chem. Soc.
, vol.193
, pp. 3081
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Meyers, A.I.1
Williams, D.R.2
Erickson, G.W.3
White, S.4
Druelinger, M.5
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17
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0001800674
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note
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288 +2351 (c 0.12, MeOH).
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18
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0001942545
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note
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288 -2483 (c 0.1, MeOH).
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