Alu-mediated PCR artifacts and the constitutional t(11;22) breakpoint

Human Molecular Genetics

Volumn 9, Issue 18, 2000, Pages 2727-2732

  Kurahashi, Hiroki ^a   Shaikh, Tamim H ^a   Emanuel, Beverly S ^a,b  

^a CHILDREN S HOSPITAL OF PHILADELPHIA   (United States)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENINE; DNA FRAGMENT; GENE PRODUCT; PALINDROMIC DNA; THYMINE;

ALU SEQUENCE; ARTICLE; ARTIFACT; CHROMOSOME 11Q; CHROMOSOME 22Q; CHROMOSOME BREAKAGE; CHROMOSOME REARRANGEMENT; CHROMOSOME TRANSLOCATION 11; CHROMOSOME TRANSLOCATION 22; CONTROLLED STUDY; DINUCLEOTIDE REPEAT; GENE AMPLIFICATION; GENE DELETION; GENE LOCATION; GENETIC ANALYSIS; GENETIC RECOMBINATION; HUMAN; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE ANALYSIS;

EID: 0034326270     PISSN: 09646906     EISSN: None     Source Type: Journal    
DOI: 10.1093/hmg/9.18.2727     Document Type: Article

References (9)

1. A procedure for in vitro amplification of DNA segments that lie outside the boundaries of known sequences
2. A novel, rapid method for the isolation of terminal sequences from yeast artificial chromosome (YAC) clones
3. Sequence specific generation of a DNA panhandle permits PCR amplification of unknown flanking DNA
4. Regions of genomic instability on 22q11 and 11q23 as the etiology for the recurrent constitutional t(11;22)
5. The most frequent constitutional translocation in humans, the t(11:22)(q23:q11) is due to a highly specific Alu-mediated recombination
6. Alu repeats and human disease
7. An in vivo assay for measuring the recombination potential between DNA sequences in mammalian cells
8. Tightly clustered 11q23 and 22q11 breakpoints permit PCR based detection of the recurrent constitutional t(11;22)
9. Effect of internal direct and inverted Alu repeat sequences on PCR