A Simplified Method for Sample Collection and DNA Isolation for Polymerase Chain Reaction Detection of Trypanosoma rangeli and Trypanosoma cruzi in Triatomine Vectors

Memorias do Instituto Oswaldo Cruz

Volumn 95, Issue 6, 2000, Pages 863-866

  Machado, Evandro M M ^a   Alvarenga, Nelson J ^a   Romanha, Alvaro J ^a   Grisard, Edmundo C ^b  

^a INSTITUTO OSWALDO CRUZ   (Brazil)

^b FEDERAL UNIVERSITY OF SANTA CATARINA   (Brazil)

Author keywords

Dipetalogaster maximus; Polymerase chain reaction; Triatomines; Trypanosoma cruzi; Trypanosoma rangeli

Indexed keywords

DIPETALOGASTER MAXIMUS; TRYPANOSOMA; TRYPANOSOMA CRUZI; TRYPANOSOMA RANGELI;

PROTOZOAL DNA;

ANIMAL; ARTICLE; DISEASE CARRIER; FECES; GENETICS; HEMOLYMPH; ISOLATION AND PURIFICATION; METHODOLOGY; PARASITOLOGY; POLYMERASE CHAIN REACTION; TRIATOMINAE; TRYPANOSOMA; TRYPANOSOMA CRUZI;

ANIMALS; DNA, PROTOZOAN; FECES; HEMOLYMPH; INSECT VECTORS; POLYMERASE CHAIN REACTION; TRIATOMINAE; TRYPANOSOMA; TRYPANOSOMA CRUZI;

EID: 0034323153     PISSN: 00740276     EISSN: None     Source Type: Journal    
DOI: 10.1590/S0074-02762000000600021     Document Type: Article

References (16)

1. Brener Z, Chiari E 1963. Variações morfológicas observadas em diferentes amostras de Trypanosoma cruzi. Rev Inst Med Trop São Paulo 5: 220-224.
2. Brenière SF, Bosseno MF, Telleria J, Carrasco R, Vargas F, Yaksic N, Noireau F 1995. Field application of polymerase chain reaction diagnosis and strain typing of Trypanosoma cruzi in Bolivian triatomines. Am J Trop Med Hyg 53: 179-184.
3. D'Alessandro A, Saravia NG 1992. Trypanosoma rangeli. In JP Kreier, JP Barker (eds), Parasitic Protozoa, 2nd ed., Academic Press Inc., San Diego, p. 1-54.
4. Diaz C, Nussenzweig V, Gonzales A 1992. An improved polymerase chain reaction assay to detect Trypanosoma cruzi in blood. Am J Trop Med Hyg 46: 616-623.
5. Grisard EC, Campbell DA, Romanha AJ 1999a. Miniexon gene sequence polymorphism among Trypanosoma rangeli strains isolated from distinct geographical regions. Parasitology 118: 375-382.
6. Grisard EC, Steindel M, Guarneri AA, Eger-Mangrich I, Campbell DA, Romanha AJ 1999b. Characterization of Trypanosoma rangeli strains isolated in Central and South America: an overview. Mem Inst Oswaldo Cruz 94: 203-209.
7. Moser DR, Kirchhoff LV, Donelson JE 1989. Detection of Trypanosoma cruzi by DNA amplification using the polymerase chain reaction. J Clin Microbiol 27: 1477-1482.
8. Pena SDJ, Barreto G, Vago AR, Marco LD, Reinach FC, Dias Neto E, Simpson AJG 1994. Sequence-specific "gene signatures" can be obtained by PCR with single specific primers at low stringency. Proc Natl Acad Sci USA 91: 1946-1949.
9. Russomando G, Rojas de Arias A, Almiron M, Figueiredo A, Ferreira ME, Morita K 1996. Trypanosoma cruzi: PCR - based detection in dried feces of Triatoma infestans. Exp Parasitol 83: 62-66.
10. Santos FR, Pena SDJ, Epplen JT 1993. Genetic and population study of an Y-linked tetranucleotide repeat DNA polymorphism. Human Genetics 90: 655-656.
11. Shikanai-Yasuda MA, Ochs DE, Tolezano JE, Kirchhoff LV 1996. Use of PCR for detecting Trypanosoma cruzi in triatomine vectors. Trans R Soc Trop Med Hyg 90: 649-651.
12. Souto RP, Vargas N, Zingales, B 1999. Trypanosoma rangeli: discrimination from Trypanosoma cruzi based on a variable domain from the large subunit ribosomal RNA gene. Exp Parasitol 91: 306-314.
13. Steindel M, Carvalho Pinto CJ, Toma HK, Mangia RHR, Ribeiro-Rodrigues R, Romanha AJ 1991. Trypanosoma rangeli (Tejera, 1920) isolated from a sylvatic rodent (Echimys dasythrix) in Santa Catarina Island, Santa Catarina State: first report of this trypanosome in Southern Brazil. Mem Inst Oswaldo Cruz 86: 73-79.
14. Steindel M, Dias Neto E, Ribeiro-Rodrigues R, Carvalho Pinto CJ, Grisard EC, Menezes CLP, Murta SMF, Simpson AJG, Romanha AJ 1994. Randomly amplified polymorphic DNA (RAPD) and isoenzyme analysis of Trypanosoma rangeli strains. J Euk Microbiol 41: 261-267.
15. Vallejo GA, Guhl F, Chiari E, Macedo AM 1999. Species specific detection of Trypanosoma cruzi and Trvpanosoma rangeli in vector and mammalian hosts by polymerase chain reaction amplification of kinetoplast minicircle DNA. Acta Trop 72: 203-212.
16. WHO - World Health Organization 1991. Control of Chagas Disease, WHO Technical Report Series 811, Geneva, 95 pp.