Optimization of Coomassie staining for quantitative densitometry of soybean storage proteins in gradient gel electrophoresis

JAOCS, Journal of the American Oil Chemists' Society

Volumn 77, Issue 12, 2000, Pages 1251-1254

  Kwanyuen, Prachuab ^a,b   Wilson, Richard F ^a  

^a NORTH CAROLINA STATE UNIVERSITY   (United States)

^b NORTH CAROLINA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DENSITOMETERS; ELECTROPHORESIS; GLYCEROL; OPTIMIZATION; POLYPEPTIDES;

COOMASSIE STAINING; GRADIENT GEL ELECTROPHORESIS;

PROTEINS;

EID: 0034318636     PISSN: 0003021X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11746-000-0196-0     Document Type: Article

References (10)

1. Kinsella, J.E., S. Damodaran, and B. German, Physicochemical and Functional Properties of Oilseed Proteins with Emphasis on Soy Proteins, in New Protein Foods, Vol. 5: Seed Storage Proteins, edited by A.M. Altschul and H.L. Wilcke, Academic Press, Orlando, 1985, pp. 107-179.
2. Utsumi, S., Plant Food Protein Engineering, in Advances in Food and Nutrition Research, edited by J.E. Kinsella, Academic Press, San Diego, 1992, Vol. 36, pp. 89-208.
3. Kwanyuen, P., R.F. Wilson, and J.W. Burton, Soybean Protein Quality, in Proceedings of the World Conference on Oilseed and Edible Oils Processing, edited by S.S. Koseoglu, K.C. Rhee, and R.F. Wilson, AOCS Press, Champaign, 1998, Vol. 1, pp. 284-289.
4. Neuhoff, V., R. Stamm, I. Pardowitz, N. Arold, W. Ehrhardt, and D. Taube, Essential Problems in Quantification of Proteins Following Colloidal Staining with Coomassie Brilliant Blue Dyes in Polyacrylamide Gels, and Their Solution, Electrophoresis 11:101-117 (1990).
5. Congdon, R.W., G.W. Muth, and A.G. Splittgerber, The Binding Interaction of Coomassie Blue with Proteins, Anal. Biochem. 213:407-413 (1993).
6. Thanh, V.H., and K. Shibasaki, Major Proteins of Soybean Seeds. A Straightforward Fractionation and Their Characterization, J. Agric. Food Chem. 24:1117-1121 (1976).
7. Chua, N.-H., Electrophoretic Analysis of Chloroplast Proteins, Methods Enzymol. 69:434-446 (1980).
8. Thanh, V.H., and K. Shibasaki, Heterogeneity of β-Conglycinin, Biochim. Biophys. Acta 439:326-328 (1976).
9. Thanh, V.H., and K. Shibasaki, Major Proteins of Soybean Seeds: Subunil Structure of β-Conglycinin, J. Agric. Food Chem. 26:692-695 (1978).
10. Nielsen, N.C., The Structure and Complexity of the IIS Polypeptides in Soybean, J. Am. Oil Chem. Soc. 62:1680-1686 (1985).