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A piezoelectric sensor was inserted into a rigid strap which was wrapped around the thorax.
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Experiments were performed on adult male Wistar rats under general anesthesia with ketaminhydrochlorid 100 mg/kg b.w. and xylazinhydrochorid 20 mg/kg b.w., administered in a bolus, intraperitoneally. During the recording the animal was placed on a homeothermic operating table that enabled the body temperature to be kept constant at 37 °C.
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The depth of anesthesia was assessed at 5-min intervals, starting from the moment at which the drugs were applied, by the animal's response to touch and to painful stimulus with the skin pinch test. The plantar surface of the animal's hind paws was stroked with the tip of a pair of tweezers (touch stimulus) or pinched (pain stimulus). The tests were considered positive if the animal responded by flexing its fingers or withdrawing its limb and were not perturbative to any of the quantities analyzed (see Figs. 2 and 3).
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