Application of directly coupled LC-NMR-MS to the structural elucidation of metabolites of the HIV-1 reverse-transcriptase inhibitor BW935U83

Journal of Chromatography B: Biomedical Sciences and Applications

Volumn 748, Issue 1, 2000, Pages 269-279

  Shockcor, John P ^a,b   Unger, Steve E ^a   Savina, Paul ^b   Nicholson, Jeremy K ^c   Lindon, John C ^c  

^a DUPONT COMPANY   (United States)

^b GLAXOSMITHKLINE   (United States)

^c IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

BW935U83; HIV 1 reverse transcriptase inhibitor; Structural elucidation

Indexed keywords

3 FLUORORIBOLACTONE; DRUG METABOLITE; GLUCURONIDE; LACTONE DERIVATIVE; RALURIDINE; RNA DIRECTED DNA POLYMERASE INHIBITOR; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARTICLE; CONJUGATE; DRUG METABOLISM; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; LIQUID CHROMATOGRAPHY; MASS SPECTROMETER; MASS SPECTROMETRY; MOLECULAR WEIGHT; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; STRUCTURE ACTIVITY RELATION;

ANTI-HIV AGENTS; CHROMATOGRAPHY, LIQUID; FURANS; HUMANS; MAGNETIC RESONANCE SPECTROSCOPY; MASS SPECTROMETRY; MOLECULAR STRUCTURE; REVERSE TRANSCRIPTASE INHIBITORS; URACIL;

EID: 0034306614     PISSN: 13872273     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-4347(00)00360-1     Document Type: Article

References (7)

1. The development and application of coupled HPLC-NMR spectroscopy
2. Direct coupling of chromatographic separations to NMR spectroscopy
3. Directly coupled HPLC-NMR and its application to drug metabolism
4. HPLC-NMR identification of the human urinary metabolites of (-)-cis-5-fluoro-1-[2-(hydroxymethyl)-1,3-oxathiolan-5-yl]-cytosi ne. A nucleoside analogue active against human immunodeficiency virus (HIV)
5. Combined hyphenation of HPLC, NMR spectroscopy and ion-trap mass spectrometry (HPLC-NMR-MS) with application to the detection and characterisation of xenobiotic and endogenous metabolites in human urine
6. On-line liquid chromatography coupled with high field NMR and mass spectrometry (LC-NMR-MS): A new technique for drug metabolite structure elucidation