Cloning and functional expression of a encoding a vacoular-type proton-translocating pyrophosphatase from Trypanosoma cruzi

Biochemical Journal

Volumn 351, Issue 1, 2000, Pages 281-288

  Hill, J E ^a   Scott, D A ^a   Luo, S ^a   Docampo, R ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

Acidocalcisome; Vacuolar pyrophosphatase; Volutin granule

Indexed keywords

COMPLEMENTARY DNA; DICYCLOHEXYLCARBODIIMIDE; INORGANIC PYROPHOSPHATASE; N ETHYLMALEIMIDE; PROTON TRANSLOCATING PYROPHOSPHATASE; UNCLASSIFIED DRUG;

ACIDIFICATION; ARTICLE; CELL VACUOLE; GENE EXPRESSION; GENE FUNCTION; GENE SEQUENCE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; TRYPANOSOMA CRUZI;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; ANIMAL; CLONING, MOLECULAR; DICYCLOHEXYLCARBODIIMIDE; DNA, COMPLEMENTARY; ENZYME INHIBITORS; ETHYLMALEIMIDE; GENOMIC LIBRARY; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PHYLOGENY; PLANTS; PROTONS; PYROPHOSPHATASES; RNA, MESSENGER; SACCHAROMYCES CEREVISIAE; SEQUENCE ALIGNMENT; SUPPORT, U.S. GOV'T, P.H.S.; TRYPANOSOMA CRUZI; VACUOLES;

SACCHAROMYCES CEREVISIAE; TRYPANOSOMA CRUZI;

EID: 0034306414     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3510281     Document Type: Article

References (33)

1. American trypanosomiasis (Chagas' disease) - A tropical disease now in the United States
2. Presence of a plant-like proton-pumping pyrophosphatase in acidocalcisomes of Trypanosoma cruzi
3. 2+ storage compartment in trypanosomatids and apicomplexan parasites
4. +-translocating pyrophosphatase
5. +-PPases: A tightly membrane-bound family
6. A thermostable vacuolar-type membrane pyrophosphatase from the archeon Pyrobaculum aerophilum: Implications for the origins of pyrophosphate-energized pumps
7. Pyrophosphate-induced acidification of trans cisternal elements of rat liver Golgi apparatus
8. Characterization of a vacuolar pyrophosphatase in Trypanosoma brucei and its localization to acidocalcisomes
9. +-ATPase
10. +-pyrophosphatase in Plasmodium falciparum
11. Vacuolar proton pyrophosphatase activity and pyrophosphate in Toxoplasma gondii as possible chemotherapeutic targets
12. Isotopes incorporated in the nucleic acids of Trypanosoma mega
13. +-pyrophosphatases
14. Cloning and expression of Trypanosoma cruzi ribosomal protein PO and epitope analysis of anti-PO autoantibodies in Chagas' disease patients
15. Improved method for high efficiency transformation of intact yeast cells
16. Molecular cloning and sequence of cDNA encoding the pyrophosphate-energized vacuolar membrane proton pump of Arabidopsis thaliana
17. +-translocating pyrophosphatase: A new category of ion transrocase
18. A site-directed mutagenesis study of Saccharomyces cerevisiae pyrophosphatase. Functional conservation of the active site of soluble inorganic pyrophosphatases
19. +-pyrophosphatase. Necessity of Cys634 for inhibition by maleimides but not catalysis
20. +-pyrophosphatase by N,N'-dicyclohexylcarbodi-imide
21. +-pyrophosphatase by N,N'-dicyclohexylcarbodiimide
22. 2+ storage in Trypanosoma brucel: The influence of cytoprasmic pH and importance of vacuolar acidity
23. Heterologous expression of plant vacuolar pyrophosphatase in yeast demonstrates sufficiency of the substrate-binding subunit for proton transport
24. Cord calcium signaling in Arabidopsis involves two cellular pools and a change in calcium signature after acclimation
25. Dicyclohexylcarbodiimide as a probe for proton translocating enzymes
26. Kingdom protozoa and its 18 phyla
27. Determining divergence times of the major kingdoms of living organisms with a protein clock
28. Cloning, sequencing, and functional activity of the Trypanosoma brucei brucei alternative oxidase
29. Herbicides to curb human parasitic infections: In vitro and in vivo effects of trifluralin on the trypanosomatid protozoans
30. Sterol biosynthesis inhibitors as potential chemotherapeutic agents against Chagas' disease
31. The molecular and biochemical basis of pyrophosphate-energized proton translocation at the vacuolar membrane