Plate assay for endo-β-N-acetylglucosaminidase activity using a chromogenic substrate synthesized by transglycosylation with Arthrobacter endo-β-N-acetylglucosaminidase

Journal of Bioscience and Bioengineering

Volumn 90, Issue 4, 2000, Pages 462-464

  Fujita, Kiyotaka ^a   Asada, Yasuhiko ^a   Yamamoto, Kenji ^b   Takegawa, Kaoru ^a  

^a KAGAWA UNIVERSITY   (Japan)

^b KYOTO UNIVERSITY   (Japan)

Author keywords

Colorimetric detection; Endo N acetylglucosaminidase; Transglycosylation; X Glc

Indexed keywords

BIOASSAY; BIOSYNTHESIS; ENZYME KINETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; MICROORGANISMS; ORGANIC SOLVENTS; POLYSACCHARIDES;

CHROMOGENIC SUBSTRATE; COLOMETRIC DETECTION; ENDO ACETYLGLUCOSAMINIDASE ACTIVITY; OLIGOSACCHARIDE; PLATE ASSAY; TRANSGLYCOSYLATION;

ENZYMES;

CHROMOGENIC SUBSTRATE; N ACETYL BETA GLUCOSAMINIDASE;

ARTHROBACTER; ARTICLE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME SUBSTRATE; ENZYME SYNTHESIS; GLYCOSYLATION; NONHUMAN;

EID: 0034302291     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1389-1723(01)80021-9     Document Type: Article

References (15)

1. Transglycosylation activity of endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae
2. 9GlcNAc to L-fucose by endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae
3. Enzymatic synthesis of novel oligosaccharides by use of transglycosylation activity of endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae
4. Enzymatic synthesis of a neoglycoconjugate by transglycosylation with Arthrobacter endo-β-N-acetylglucosaminidase: A substrate for colorimetric detection of endo-β-N-acetylglucosaminidase activity
5. Synthesis of neoglycoconjugates by transglycosylation with Arthrobacter protophormiae endo-β-N-acetylglucosaminidase. Demonstration of a macro-cluster effect for mannose-binding proteins
6. Synthesis of neoglycoproteins using oligosaccharide-transfer activity with endo-β-N-acetylglucosaminidase
7. Chemo-enzymatic synthesis of a calcitonin derivative containing a high-mannose type oligosaccharide by endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae
8. Synthesis of neoglycoenzymes with homogeneous N-linked oligosaccharides using immobilized endo-β-N-acetylglucosaminidase
9. Enhanced transglycosylation activity of Arthrobacter protophormiae endo-β-N-acetylglucosaminidase in media containing organic solvents
10. Separation of glycoprotein-derived oligosaccharides by thin-layer chromatography
11. Complete amino acid sequence of endo-β-N-acetylglucosaminidase from Flavobacterium sp.
12. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid
13. Cloning and nucleotide sequence of the β-D-glucosidase gene from Bifidobacterium breve clb, and expression of β-D-glucosidase activity in Escherichia coli
14. Cloning, sequencing, and expression of Arthrobacter protophormiae endo-β-N-acetylglucosaminidase in Escherichia coli
15. Analysis of endo-β-N-acetylglucosaminidase activity by high-pressure liquid chromatography on a silica-based chemically bonded octadecyl column