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The protein is probably denatured by a process of energy transfer from the pyrene derivatives to the host lysozyme molecules, leading to denaturation and eventual fragmentation. An enlightening study of analogous photochemical splicing of lysozyme in solution by pyrenes has been described recently by: Kumar, C. V.; Buranaprapuk, A.; Opiteck, G. J.; Moyer, M. B.; Jockusch, S.; Turro, N. J. Proc. Natl. Acad. Sci. U.S.A. 1998, 95, 10361.
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The following files from the Brookhaven Protein Data Bank were used: 1AKI (orthorhombic) and 6LYZ (tetragonal).
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The following files from the Brookhaven Protein Data Bank were used: 1AKI (orthorhombic) and 6LYZ (tetragonal).
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a value should be similar to that of propylamine, viz., ca. 10.7: CRC Handbook of Chemistry and Physics; Weast, R. C., Ed.; CRC Press: Boca Raton, FL, 1995.
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