Lanthanide Ions Bind Specifically to an Added "EF-Hand" and Orient a Membrane Protein in Micelles for Solution NMR Spectroscopy

Journal of Magnetic Resonance

Volumn 146, Issue 2, 2000, Pages 381-384

  Ma, Che ^a,b   Opella, Stanley J ^a,b  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Lanthanide; Membrane protein; Micelles; Residual dipolar couplings; Vpu

Indexed keywords

LANTHANIDE; MEMBRANE PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; HUMAN IMMUNODEFICIENCY VIRUS 1; METHODOLOGY; MICELLE; MOLECULAR GENETICS; NUCLEAR MAGNETIC RESONANCE;

AMINO ACID SEQUENCE; HIV-1; MEMBRANE PROTEINS; METALS, RARE EARTH; MICELLES; MOLECULAR SEQUENCE DATA; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR;

EID: 0034293196     PISSN: 10907807     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmre.2000.2172     Document Type: Editorial

References (16)

1. N. Tjandra and A. Bax, Direct measurement of distances and angles in biomolecules by NMR in a dilute liquid crystalline medium. Science 278, 1111-1114 (1997).
2. G. M. Clore, M. R. Starich, and A. M. Gronenborn, Measurements of residual dipolar couplings of macromolecules aligned in the nematic phase of a colloidal suspension of rod-shaped viruses. J. Am. Chem. Soc. 120, 10571-10572 (1998).
3. M. R. Hansen, L. Mueller, and A. Pardi, Tunable alignment of macromolecules by filamentous phage yields dipolar coupling interactions. Nat. Struct. Biol. 5, 1065-1074 (1998).
4. J. R. Tolman, J. M. Flanagan, M. A. Kennedy, and J. H. Prestegard, Nuclear magnetic dipole interactions in field-oriented proteins - Information for structure determination in solution. Proc. Natl. Acad. Sci. USA 92, 9279-9283 (1995).
5. M. A. Contreras, J. Ubach, O. Millet, J. Rizo, and M. Pons, Measurement of one bond dipolar couplings through lanthanide-induced orientation of a calcium-binding protein. J. Am. Chem. Soc. 121, 8947-8948 (1999).
6. A. Tuechelmann, H. Schwalbe, and C. Griesinger, Magnetically anisotropic tags for biomolecules. Abstract, 3rd European Conference, Oxford (1998).
7. V. V. Gaponenko, C. J. Walsey, B. M. Hoffman, and P. R. Rosevear, Determination of residual dipolar couplings using a cobalt binding tag. Biophys. J. 78, 1705Pos (2000).
8. M. Allegrozzi, I. Bertini, M. B. L. Janik, Y. M. Lee, G. H. Lin, and C. Luchinat, Lanthanide-induced pseudocontact shifts for solution structure refinements of macromolecules in shells up to 40 angstrom from the metal ion. J. Am. Chem. Soc. 122, 4154-4161 (2000).
9. H. M. Al-Hashimi, H. Valafar, M. Terrell, E. R. Zartler, M. K. Eidsness, and J. H. Prestegard, Variation of molecular alignment as a means of resolving orientational ambiguities in protein structures from dipolar couplings. J. Magn. Reson. 143, 402-406 (2000).
10. K. Strebel, T. Klimkait, F. Maldarelli, and M. A. Martin, Molecular and biochemical analyses of human immunodeficiency virus type 1 vpu protein. J. Virol. 63, 3784-3791 (1989).
11. R. L. Willey, F. Maldarelli, M. A. Martin, and K. Strebel, Human immunodeficiency virus type 1 Vpu protein induces rapid degradation of CD4. J. Virol. 66, 7193-7200 (1992).
12. F. M. Marassi, C. Ma, H. Gratkowski, S. K. Straus, K. Strebel, M. Oblatt-Montal, M. Montal, and S. J. Opella, Correlation of the structural and functional domains in the membrane protein Vpu from HIV-1. Proc. Natl. Acad. Sci. USA 96, 14336-14341 (1999).
13. T. C. Williams, D. C. Corson, and B. D. Sykes, Calcium-binding proteins: Calcium(II)-lanthanide(III) exchange in carp parvalbumin. J. Am. Chem. Soc. 106, 5698-5702 (1984).
14. J.-C. Hus, D. Marion, and M. Blackledge, De novo determination of protein structure by NMR using orientational and long-range order restraints. J. Mol. Biol. 298, 927-936 (2000).
15. F. R. Ahmed, D. R. Rose, S. V. Evans, M. E. Pippy, and R. To, Refinement of recombinant oncomodulin at 1.30-angstrom resolution. J. Mol. Biol. 230, 1216-1224 (1993).
16. M. Ottiger, F. Delaglio, and A. Bax, Measurement of J and dipolar couplings from simplified two-dimensional NMR spectra. J. Magn. Reson. 131, 373-378 (1998).