Dissociation of G-protein α from rhabdomeric membranes decreases its interaction with rhodopsin and increases its degradation by calpain

Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

Volumn 127, Issue 1, 2000, Pages 75-83

  Bamsey, Christine ^a   Mayeenuddin, Linnia H ^a   Cheung, Ricky ^a   Mitchell, Jane ^a  

^a UNIVERSITY OF TORONTO   (Canada)

Author keywords

Calpain; GTP binding protein; Phospholipase C; Photoreceptor; Rhodopsin; Squid

Indexed keywords

CALPAIN; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE TRIPHOSPHATE; PHOSPHOLIPASE C; PROTEIN SUBUNIT; RHODOPSIN;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DISSOCIATION; ENZYME ACTIVATION; LIGHT ADAPTATION; NONHUMAN; PHOTOACTIVATION; PHOTORECEPTOR MEMBRANE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DEGRADATION; PROTEIN ISOLATION; PROTEIN PROTEIN INTERACTION;

ANIMALIA; CEPHALOPODA; INVERTEBRATA;

EID: 0034283434     PISSN: 03050491     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0305-0491(00)00238-8     Document Type: Article

References (33)

1. Bloomquist, B.T., Shortridge, R.D., Schneuwly, S., Perdew, M., Montell, C., Steller, H., Rubin, G., Pak, W.L., 1988. Isolation of a putative phospholipase C gene of Drosophila, norpA, and its role in phototransduction. Cell 54, 723-733.
2. Carne, A., McGregor, R.A., Bhatia, J., Sivaprasadarao, A., Kenn, J.N., Davies, A., Findlay, J.B.C., 1995. A β-subclass phosphatidylinositol-specific phospholipase C from squid (Loligo forbesi) photoreceptors exhibiting a truncated C-terminus. FEBS Lett. 372, 243-248.
3. Cincotta, D.E., Hubbell, W.L., 1977. Freeze fracture studies of squid photoreceptor membranes. Invest. Ophthalmol. Vis. Sci. (Suppl), 16.
4. Degtyarev, M.Y., Spiegel, A.M., Jones, T.L.Z., 1993. Increased palmitoylation of the Gs protein α subunit after activation by the β-adrenergic receptor or cholera toxin. J. Biol. Chem. 268, 23769-23772.
5. Fawzi, A.B., Northup, J.K., 1990. Guanine nucleotide binding characteristics of transducin: essential role of rhodopsin for rapid exchange of guanine nucleotides. Biochemistry 29, 3804-3812.
6. Fung, B.K., Lieberman, B.S., Lee, R.H., 1992. A third form of the G protein β subunit. 2. Purification and biochemical properties. J. Biol. Chem. 267, 24782-24788.
7. Graf, R., Mattera, R., Codina, J., Estes, M.K., Birnbaumer, L., 1992. A truncated recombinant α subunit of Gi3 with a reduced affinity for βγ dimers and altered guanosine-3′-o (thio)triphosphate binding. J. Biol. Chem. 267, 24037-24044.
8. Greenwood, A.F., Jope, R.S., 1994. Brain G-protein proteolysis by calpain: enhancement by lithium. Brain Res. 636, 320-326.
9. Hall, M.D., Hoon, M.A., Ryba, N.J.P., Pottinger, J.D.D., Keen, J.N., Saibil, H.R., Findlay, J.B.C., 1991. Molecular cloning and primary structure of squid (Loligo forbesi) rhodopsin, a phospholipase C-directed G-protein-linked receptor. Biochem. J. 274, 35-40.
10. 2+ channel in Drosophila photoreceptors. Neuron 8, 643-651.
11. Kikkawa, S., Tominaga, K., Nakagawa, M., Iwasa, T., Tseuda, M., 1996. Simple purification and functional reconstitution of octopus photoreceptor Gq, which couples rhodopsin to phospholipase C. Biochemistry 35, 15857-15864.
12. Kokame, K., Fukada, Y., Yoshizawa, T., Takao, T., Shimonishi, Y., 1992. Lipid modification at the N-terminus of photoreceptor G-protein α subunit. Nature 359, 749-752.
13. Laemmli, U.K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage. Nature 227, 680-685.
14. Lee, Y.J., Dobbs, M.B., Verardi, M.L., Hyde, D.R., 1990. The Drosophila dgq gene encodes a G α protein that mediates phototransduction. Neuron 5, 889-898.
15. Lott, J.S., Ryba, N.J.P., Pottinger, J.D.D., Keen, J.N., Carne, A., Findlay, J.B.C., 1992. The γ-subunit of the principal G-protein from squid (Loligo forbesi) photoreceptors contains a novel N-terminal sequence. FEBS Lett. 312, 241-244.
16. Mitchell, J., Gutierrez, J., Northup, J.K., 1995. Purification, characterization and partial amino acid sequence of a G protein-activated phospholipase C from squid photoreceptors. J. Biol. Chem. 270, 854-859.
17. Mumby, S.M., Kleuss, C., Gilman, A.G., 1994. Receptor regulation of G-protein palmitoylation. Proc. Natl. Acad. Sci. USA 91, 2800-2804.
18. Narita, K., Suzuki, T., Ohtsu, K., Seidou, M., Kito, Y., Tsukahara, Y., 1999. Structural and functional differences of two forms of GTP-binding protein, Gq, in the cephalopod retina. Comp. Biochem. Physiol. 123B, 319-327.
19. Nashima, K., Mitsudo, M., Kito, Y., 1979. Molecular weight and structural studies on cephalopod rhodopsin. Biochem. Biophys. Acta 579, 155-168.
20. Northup, J.K., Smigel, M.D., Gilman, A.G., 1982. The guanine nucleotide activating site of the regulatory component of adenylate cyclase identification of ligand binding. J. Biol. Chem. 257, 11416-11423.
21. Oldenburg, K.R., Hubbell, W.L., 1990. Invertebrate rhodopsin cleavage by an endogenous calcium activated protease. Exp. Eye Res. 51, 463-472.
22. Raaflaub, J., 1960. Applications of metal buffers and metal indicators in biochemistry. Methods Biochem. Anal. 3, 301-325.
23. Ryba, N.J.P., Pottinger, J.D.D., Keen, J.N., Findlay, J.B.C., 1991. Sequence of the β-subunit of the phosphatidylinositol-specific phospholipase C-directed GTP-binding protein from squid (Loligo forbesi) photoreceptors. Biochem. J. 273, 225-228.
24. Ryba, N.J.P., Findlay, J.B.C., Reid, J.D., 1993. The molecular cloning of the squid (Loligo forbesi) visual Gq-α subunit and its expression in Saccharomyces cerevisiae. Biochem. J. 292, 333-341.
25. Schaffner, W., Weissmann, C., 1973. A rapid, sensitive, and specific method for the determination of protein in dilute solution. Anal. Biochem. 56, 502-514.
26. Suzuki, T., Terakita, A., Narita, K., Nagai, K., Tsukahara, Y., Kito, Y., 1995. Squid photoreceptor phospholipase C is stimulated by membrane Gq α but not soluble Gq α FEBS Lett. 377, 333-337.
27. Suzuki, T., Narita, K., Terakita, A., Takai, E., Nagai, K., Kito, Y., Tsukahara, Y., 1999. Regulation of squid visual phospholipase C by activated G-protein α. Comp. Biochem. Physiol. 122A, 369-374.
28. q in crayfish photoreceptors. Vis. Neurosci. 13, 539-547.
29. Wedegaertner, P.B., Chu, D.H., Wilson, P.T., Levis, M.J., Bourne, H.R., 1993. Palmitoylation is required for signalling functions and membrane attachment of Gq α and Gs α. J. Biol. Chem. 268, 25001-25008.
30. Wedegaertner, P.B., Wilson, P.T., Bourne, H.R., 1995. Lipid modification of trimeric G proteins. J. Biol. Chem. 270, 503-506.
31. Yarfitz, S., Hurley, J.B., 1994. Transduction mechanisms of vertebrate and invertebrate photoreceptors. J. Biol. Chem. 269, 14329-14332.
32. Zucker, C.S., 1996. The biology of vision of drosophila. Proc. Natl. Acad. Sci. USA 93, 571-576.
33. Zucker, C.S., Cowman, A.F., Rubin, G.M., 1985. Isolation and structure of rhodopsin gene from D. melanogaster. Cell 40, 851-858.